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. 1971 Oct;68(10):2540–2544. doi: 10.1073/pnas.68.10.2540

Characterization of the Inducer Required for the Development of Macrophage and Granulocyte Colonies

Tamar Landau 1, Leo Sachs 1
PMCID: PMC389464  PMID: 4332814

Abstract

A substance produced by various types of cells can induce from single hematopoietic cells the formation of colonies of normal macrophages and granulocytes. This inducer has been purified 600-fold from serum-free conditioned medium from a tissue culture line of mouse cells. It is shown that the inducer is a protein, with a molecular weight of 65-70,000, whose inducing activity was about 1 ng per colony. Acrylamide gel electrophoresis of this material gave four bands, only one of which contained inducing activity. The purified protein was inactive. Activity was regained by the addition of a low molecular weight cofactor that is present in conditioned medium. Under certain conditions, activity was also regained by the addition of adenosine 3′:5′-cyclic monophosphate (cyclic AMP).

Keywords: hematopoietic, cofactor, cyclic AMP, ultrafiltration, protein inducer

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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