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. 1971 Oct;68(10):2564–2568. doi: 10.1073/pnas.68.10.2564

Lateral Diffusion of Phospholipids in a Vesicle Membrane

Roger D Kornberg 1, Harden M McConnell 1
PMCID: PMC389469  PMID: 4332815

Abstract

Nuclear-resonance spectra of phosphatidylcholine (PC) vesicles are broadened by spin-labeled PC. The broadening of the N-methyl proton line is proportional to spin-labeled PC concentration; the broadening by 1 molecule in 100 of spin-labeled PC is 9.7 Hz at 35°C; the broadening is not due to collisions between vesicles, exchange of spin label between vesicles, or fusion of vesicles. These findings are proof of rapid diffusion in the plane of the PC bilayer (lateral diffusion). A further study of the broadening reveals the molecular frequency of the translation step for lateral diffusion to be much greater than 3 × 103 sec-1 at 0°C.

Keywords: spin-labeled phosphatidylcholine, nuclear resonance

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATTWOOD D., SAUNDEES L. A LIGHT-SCATTERING STUDY OF ULTRASONICALLY IRRADIATED LECITHIN SOLS. Biochim Biophys Acta. 1965 Apr 5;98:344–350. doi: 10.1016/0005-2760(65)90126-8. [DOI] [PubMed] [Google Scholar]
  2. Chapman D., Morrison A. Physical studies of phospholipids. IV. High resolution nuclear magnetic resonance spectra of phospholipids and related substances. J Biol Chem. 1966 Nov 10;241(21):5044–5052. [PubMed] [Google Scholar]
  3. Cubero Robles E., van den Berg D. Synthesis of lecithins by acylation of O-(sn-glycero-3-phosphoryl) choline with fatty acid anhydrides. Biochim Biophys Acta. 1969 Dec 17;187(4):520–526. doi: 10.1016/0005-2760(69)90049-6. [DOI] [PubMed] [Google Scholar]
  4. Huang C. Studies on phosphatidylcholine vesicles. Formation and physical characteristics. Biochemistry. 1969 Jan;8(1):344–352. doi: 10.1021/bi00829a048. [DOI] [PubMed] [Google Scholar]
  5. Kornberg R. D., McConnell H. M. Inside-outside transitions of phospholipids in vesicle membranes. Biochemistry. 1971 Mar 30;10(7):1111–1120. doi: 10.1021/bi00783a003. [DOI] [PubMed] [Google Scholar]
  6. Poole A. R., Howell J. I., Lucy J. A. Lysolecithin and cell fusion. Nature. 1970 Aug 22;227(5260):810–814. doi: 10.1038/227810a0. [DOI] [PubMed] [Google Scholar]
  7. Sheard B. Internal mobility in phospholipids. Nature. 1969 Sep 6;223(5210):1057–1059. doi: 10.1038/2231057a0. [DOI] [PubMed] [Google Scholar]
  8. Small D. M. Phase equilibria and structure of dry and hydrated egg lecithin. J Lipid Res. 1967 Nov;8(6):551–557. [PubMed] [Google Scholar]
  9. de Gier J., Mandersloot J. G., van Deenen L. L. Lipid composition and permeability of liposomes. Biochim Biophys Acta. 1968 Jun 11;150(4):666–675. doi: 10.1016/0005-2736(68)90056-4. [DOI] [PubMed] [Google Scholar]

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