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. 1971 Oct;68(10):2589–2593. doi: 10.1073/pnas.68.10.2589

Expression of Tyrosine Aminotransferase Activity in Somatic-Cell Heterokaryons: Evidence for Negative Control of Enzyme Expression

E Brad Thompson *, T D Gelehrter †
PMCID: PMC389474  PMID: 4109409

Abstract

Cells of the tyrosine aminotransferase (EC 2.6.1.5)-inducible line, HTC, were fused with those of the BRL-62 line, which contain little or no enzyme and are unresponsive to the usual steroid inducers. Histochemical examination of the resulting heterokaryons within 24 hr of the fusion showed them to have little or no enzyme and to be unresponsive to steroids. These experiments disclose that the observed negative dominance is universal in the fused cells and that such dominance occurs promptly after fusion, when loss of chromosomes has not occurred.

Keywords: rat, cell culture, cell fusion, autoradiography, histochemical staining

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auricchio F., Martin D., Jr, Tompkins G. Control of degradation and synthesis of induced tyrosine aminotransferase studied in hepatoma cells in culture. Nature. 1969 Nov 22;224(5221):806–808. doi: 10.1038/224806b0. [DOI] [PubMed] [Google Scholar]
  2. Ballard P. L., Tomkins G. M. Hormone induced modification of the cell surface. Nature. 1969 Oct 25;224(5217):344–345. doi: 10.1038/224344a0. [DOI] [PubMed] [Google Scholar]
  3. Baxter J. D., Tomkins G. M. The relationship between glucocorticoid binding and tyrosine aminotransferase induction in hepatoma tissue culture cells. Proc Natl Acad Sci U S A. 1970 Mar;65(3):709–715. doi: 10.1073/pnas.65.3.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davidson R. L. Regulation of gene expression in somatic cell hybrids: a review. In Vitro. 1971 May-Jun;6(6):411–426. doi: 10.1007/BF02616043. [DOI] [PubMed] [Google Scholar]
  5. Davidson R., Yamamoto K. Regulation of melanin synthesis in mammalian cells, as studied by somatic hybridization. II. The level of regulation of 3,4-dihydroxyphenylalanine oxidase. Proc Natl Acad Sci U S A. 1968 Jul;60(3):894–901. doi: 10.1073/pnas.60.3.894. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gelehrter T. D., Tomkins G. M. The role of RNA in the hormonal induction of tyrosine aminotransferase in mammalian cells in tissue culture. J Mol Biol. 1967 Oct 14;29(1):59–76. doi: 10.1016/0022-2836(67)90181-7. [DOI] [PubMed] [Google Scholar]
  7. Granner D. K., Hayashi S., Thompson E. B., Tomkins G. M. Stimulation of tyrosine aminotransferase synthesis by dexamethasone phosphate in cell culture. J Mol Biol. 1968 Jul 28;35(2):291–301. doi: 10.1016/s0022-2836(68)80025-7. [DOI] [PubMed] [Google Scholar]
  8. Granner D. K., Thompson E. B., Tomkins G. M. Dexamethasone phosphate-induced synthesis of tyrosine aminotransferase in hepatoma tissue culture cells. Studies of the early phases of induction and of the steroid requirement for maintanance of the induced rate of synthesis. J Biol Chem. 1970 Mar 25;245(6):1472–1478. [PubMed] [Google Scholar]
  9. Harris H., Cook P. R. Synthesis of an enzyme determined by an erythrocyte nucleus in a hybrid cell. J Cell Sci. 1969 Jul;5(1):121–133. doi: 10.1242/jcs.5.1.121. [DOI] [PubMed] [Google Scholar]
  10. Harris H., Sidebottom E., Grace D. M., Bramwell M. E. The expression of genetic information: a study with hybrid animal cells. J Cell Sci. 1969 Mar;4(2):499–525. doi: 10.1242/jcs.4.2.499. [DOI] [PubMed] [Google Scholar]
  11. Hayashi S. I., Granner D. K., Tomkins G. M. Tyrosine aminotransferase. Purificaton and characterization. J Biol Chem. 1967 Sep 25;242(18):3998–4006. [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Martin D. W., Jr, Tomkins G. M., Bresler M. A. Control of specific gene expression examined in synchronized mammalian cells. Proc Natl Acad Sci U S A. 1969 Jul;63(3):842–849. doi: 10.1073/pnas.63.3.842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Peterkofsky B., Tomkins G. M. Effect of inhibitors of nucleic acid synthesis on steroid-mediated induction of tyrosine aminotransferase in hepatoma cell cultures. J Mol Biol. 1967 Nov 28;30(1):49–61. doi: 10.1016/0022-2836(67)90242-2. [DOI] [PubMed] [Google Scholar]
  15. Reel J. R., Kenney F. T. "Superinduction" of tyrosine transaminase in hepatoma cell cultures: differential inhibition of synthesis and turnover by actionomycin D. Proc Natl Acad Sci U S A. 1968 Sep;61(1):200–206. doi: 10.1073/pnas.61.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reel J. R., Lee K. L., Kenney F. T. Regulation of tyrosine alpha-ketoglutarate transaminase in rat liver. 8. Inductions by hydrocortisone and insulin in cultured hepatoma cells. J Biol Chem. 1970 Nov 10;245(21):5800–5805. [PubMed] [Google Scholar]
  17. Samuels H. H., Tomkins G. M. Relation of steroid structure to enzyme induction in hepatoma tissue culture cells. J Mol Biol. 1970 Aug 28;52(1):57–74. doi: 10.1016/0022-2836(70)90177-4. [DOI] [PubMed] [Google Scholar]
  18. Schimke R. T. Protein turnover and the regulation of enzyme levels in rat liver. Natl Cancer Inst Monogr. 1967 Nov;27:301–314. [PubMed] [Google Scholar]
  19. Schneider J. A., Weiss M. C. Expression of differentiated functions in hepatoma cell hybrids. I. Tyrosine aminotransferase in hepatoma-fibroblast hybrids. Proc Natl Acad Sci U S A. 1971 Jan;68(1):127–131. doi: 10.1073/pnas.68.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Silagi S. Hybridization of a malignant melanoma cell line with L cells in vitro. Cancer Res. 1967 Nov;27(11):1953–1960. [PubMed] [Google Scholar]
  21. Subak-Sharpe H., Bürk R. R., Pitts J. D. Metabolic co-operation between biochemically marked mammalian cells in tissue culture. J Cell Sci. 1969 Mar;4(2):353–367. doi: 10.1242/jcs.4.2.353. [DOI] [PubMed] [Google Scholar]
  22. Thompson E. B., Granner D. K., Tomkins G. M. Superinduction of tyrosine aminotransferase by actinomycin D in rat hepatoma (HTC) cells. J Mol Biol. 1970 Dec 14;54(2):159–175. doi: 10.1016/0022-2836(70)90424-9. [DOI] [PubMed] [Google Scholar]
  23. Thompson E. B., Tomkins G. M. A histochemical method for the demonstration of tyrosine aminotransferase in tissue culture cells, and studies of this enzyme in hepatoma tissue culture cells. J Cell Biol. 1971 Jun;49(3):921–927. doi: 10.1083/jcb.49.3.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Thompson E. B., Tomkins G. M., Curran J. F. Induction of tyrosine alpha-ketoglutarate transaminase by steroid hormones in a newly established tissue culture cell line. Proc Natl Acad Sci U S A. 1966 Jul;56(1):296–303. doi: 10.1073/pnas.56.1.296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tomkins G. M., Gelehrter T. D., Granner D., Martin D., Jr, Samuels H. H., Thompson E. B. Control of specific gene expression in higher organisms. Expression of mammalian genes may be controlled by repressors acting on the translation of messenger RNA. Science. 1969 Dec 19;166(3912):1474–1480. doi: 10.1126/science.166.3912.1474. [DOI] [PubMed] [Google Scholar]
  26. Tomkins G. M., Thompson E. B., Hayashi S., Gelehrter T., Granner D., Peterkofsky B. Tyrosine transaminase induction in mammalian cells in tissue culture. Cold Spring Harb Symp Quant Biol. 1966;31:349–360. doi: 10.1101/sqb.1966.031.01.045. [DOI] [PubMed] [Google Scholar]

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