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. 1971 Nov;68(11):2674–2677. doi: 10.1073/pnas.68.11.2674

Hormonal Control of Lecithin Synthesis in Barley Aleurone Cells: Regulation of the CDP-Choline Pathway by Gibberellin

Kenneth D Johnson 1,*, Hans Kende 1
PMCID: PMC389498  PMID: 16591952

Abstract

The enzymes of the cytidine diphosphate-choline pathway, which is involved in lecithin biosynthesis, are present in imbibed barley aleurone cells. The first enzyme, choline kinase (EC 2.7.1.32), is found in the soluble protein fraction. Its activity is not affected by prior treatment of aleurone layers with gibberellin. The second and third enzymes of the pathway, phosphorylcholine-cytidyl (EC 2.7.7.15) and phosphorylcholine-glyceride (EC 2.7.8.2) transferases, are associated with the particulate fractions. Their activities are greatly increased by gibberellin treatment during the lag phase (0-8 hr) of gibberellin-effected α-amylase synthesis. The hormonal effects are evident two hours after gibberellin treatment. Inhibitors that block gibberellin-effected α-amylase formation also inhibit the stimulation of these membrane-bound enzymes by the hormone.

Keywords: membrane synthesis, rough endoplasmic reticulum, enzyme induction, abscisic acid, actinomycin D

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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