Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Nov;68(11):2694–2698. doi: 10.1073/pnas.68.11.2694

Specific Binding of Peroxidase-Labeled Myelin Basic Protein in Allergic Encephalomyelitis

Anne B Johnson *, Henryk M Wiśniewski *, Cedric S Raine *, E H Eylar †,†, Robert D Terry *
PMCID: PMC389503  PMID: 5288245

Abstract

A conjugate of horseradish peroxidase and the encephalitogenic basic protein from myelin has been used to study the antigen reactivity of tissue in the autoimmune disease, experimental allergic encephalomyelitis. Control conjugates were also prepared of peroxidase and bovine serum albumin and of peroxidase and lysozyme, another basic protein. The basic protein from myelin conjugate was specifically bound by lymph node cells from rabbits immunized against the basic protein. Some of these cells appeared to be plasma cells. The conjugate was also specifically bound by occasional cells in the spinal-cord infiltrates of animals with early signs of allergic encephalomyelitis. These cells resembled large lymphocytes and plasma cells. There was no difference between the binding of basic protein of bovine and rabbit origin. The findings suggest the possibility that a local release of antibody within the target organ may play a role in the pathogenesis of allergic encephalomyelitis.

Keywords: antigen, antibody, autoimmune, immunopathology, histochemistry

Full text

PDF
2694

Images in this article

2695Image
on p.2695
2697Image
on p.2697

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry. 1969 Jan;6(1):43–52. doi: 10.1016/0019-2791(69)90177-3. [DOI] [PubMed] [Google Scholar]
  2. Avrameas S., Leduc E. H. Detection of simultaneous antibody synthesis in plasma cells and specialized lymphocytes in rabbit lymph nodes. J Exp Med. 1970 Jun 1;131(6):1137–1168. doi: 10.1084/jem.131.6.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Avrameas S., Ternynck T. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969 Jan;6(1):53–66. doi: 10.1016/0019-2791(69)90178-5. [DOI] [PubMed] [Google Scholar]
  4. Behnke O. Demonstration of endogenous peroxidase activity in the electron microscope. J Histochem Cytochem. 1969 Jan;17(1):62–64. doi: 10.1177/17.1.62. [DOI] [PubMed] [Google Scholar]
  5. Budzko D. B., Müller-Eberhard H. Anaphylatoxin release from the third component of human complement by hydroxylamine. Science. 1969 Aug 1;165(3892):506–507. doi: 10.1126/science.165.3892.506. [DOI] [PubMed] [Google Scholar]
  6. Burnett P. R., Eylar E. H. Allergic encephalomyelitis. Oxidation and cleavage of the single tryptophan residue of the A1 protein from bovine and human myelin. J Biol Chem. 1971 May 25;246(10):3425–3430. [PubMed] [Google Scholar]
  7. Carnegie P. R. Properties, structure and possible neuroreceptor role of the encephalitogenic protein of human brain. Nature. 1971 Jan 1;229(5279):25–28. doi: 10.1038/229025a0. [DOI] [PubMed] [Google Scholar]
  8. Dowling P. C., Cook S. D. Pattern of cellular and humoral events in experimental allergic encephalomyelitis. Neurology. 1968 Oct;18(10):953–962. doi: 10.1212/wnl.18.10.953. [DOI] [PubMed] [Google Scholar]
  9. Eylar E. H., Salk J., Beveridge G. C., Brown L. V. Experimental allergic encephalomyelitis. An encephalitogenic basic protein from bovine myelin. Arch Biochem Biophys. 1969 Jun;132(1):34–48. doi: 10.1016/0003-9861(69)90336-1. [DOI] [PubMed] [Google Scholar]
  10. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  11. Lampert P. Electron microscopic studies on ordinary and hyperacute experimental allergic encephalomyelitis. Acta Neuropathol. 1967 Oct 20;9(2):99–126. doi: 10.1007/BF00691436. [DOI] [PubMed] [Google Scholar]
  12. Lennon V. A., Wilks A. V., Carnegie P. R. Immunologic properties of the main encephalitogenic peptide from the basic protein of human myelin. J Immunol. 1970 Nov;105(5):1223–1230. [PubMed] [Google Scholar]
  13. Levine S., Hoenig E. M., Kies M. W. Allergic encephalomyelitis: passive transfer prevented by encephalitogen. Science. 1968 Sep 13;161(3846):1155–1157. doi: 10.1126/science.161.3846.1155. [DOI] [PubMed] [Google Scholar]
  14. MCCLUSKEY R. T., BENACERRAF B., MCCLUSKEY J. W. STUDIES ON THE SPECIFICITY OF THE CELLULAR INFILTRATE IN DELAYED HYPERSENSITIVITY REACTIONS. J Immunol. 1963 Mar;90:466–477. [PubMed] [Google Scholar]
  15. MacLennan I. C., Harding B. The role of immunoglobulins in lymphocyte-mediated cell damage, in vitro. II. The mechanism of target cell damage by lymphoid cells from immunized rats. Immunology. 1970 Mar;18(3):405–412. [PMC free article] [PubMed] [Google Scholar]
  16. Modabber F., Morikawa S., Coons A. H. Antigen-binding cells in normal mouse thymus. Science. 1970 Dec 4;170(3962):1102–1104. doi: 10.1126/science.170.3962.1102. [DOI] [PubMed] [Google Scholar]
  17. NAJARIAN J. S., FELDMAN J. D. SPECIFICITY OF PASSIVELY TRANSFERRED DELAYED HYPERSENSITIVITY. J Exp Med. 1963 Sep 1;118:341–352. doi: 10.1084/jem.118.3.341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Oldstone M. B., Dixon F. J. Immunohistochemical study of allergic encephalomyelitis. Am J Pathol. 1968 Feb;52(2):251–263. [PMC free article] [PubMed] [Google Scholar]
  19. RAUCH H. C., RAFFEL S. ANTIGEN UPTAKE BY SPECIFICALLY REACTIVE CELLS IN EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS. Ann N Y Acad Sci. 1965 Mar 31;122:297–307. doi: 10.1111/j.1749-6632.1965.tb20214.x. [DOI] [PubMed] [Google Scholar]
  20. Turk J. L. Cytology of the induction of hypersensitivity. Br Med Bull. 1967 Jan;23(1):3–8. doi: 10.1093/oxfordjournals.bmb.a070511. [DOI] [PubMed] [Google Scholar]
  21. Wiśniewski H., Prineas J., Raine C. S. An ultrastructural study of experimental demyelination and remyelination. I. Acute experimental allergic encephalomyelitis in the peripheral nervous system. Lab Invest. 1969 Aug;21(2):105–118. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES