Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Nov;68(11):2757–2760. doi: 10.1073/pnas.68.11.2757

Regulation of Adenosine 3′:5′-Cyclic Monophosphate Concentration in Cultured Human Astrocytoma Cells by Catecholamines and Histamine

Richard B Clark 1, John P Perkins 1
PMCID: PMC389518  PMID: 4330940

Abstract

Norepinephrine, epinephrine, and histamine cause a rapid increase in the concentration of adenosine 3′:5′-cyclic monophosphate (cAMP) in a tumor astrocyte cell line derived from a primary culture of a human glioblastoma multiforme. The catecholamine-induced increase in cAMP is dependent on the cell density, being far greater in cells in the log phase of growth than in cells near terminal density. The response to norepinephrine is inhibited 50% by 0.01 μM propranolol, a blocking agent of β-adrenergic receptors. In contrast, the effect of histamine on cAMP concentration varies only slightly from log-phase growth to terminal density, and is not inhibited by 10 μM propranolol. The results suggest that astrocytoma cells have independent receptors for catecholamines and histamine. Further, if the astrocytoma cell is an adequate model of the normal glial cell, these results suggest that astrocytes in human cerebral cortex may be sensitive to norepinephrine and histamine.

Keywords: growth cycle, propranolol, theophylline

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUTCHER R. W., SUTHERLAND E. W. Adenosine 3',5'-phosphate in biological materials. I. Purification and properties of cyclic 3',5'-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3',5'-phosphate in human urine. J Biol Chem. 1962 Apr;237:1244–1250. [PubMed] [Google Scholar]
  2. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Huang M., Shimizu H., Daly J. Regulation of adenosine cyclic 3',5'-phosphate formation in cerebral cortical slices. Interaction among norepinephrine, histamine, serotonin. Mol Pharmacol. 1971 Mar;7(2):155–162. [PubMed] [Google Scholar]
  4. Kakiuchi S., Rall T. W., McIlwain H. The effect of electrical stimulation upon the accumulation of adenosine 3',5'-phosphate in isolated cerebral tissue. J Neurochem. 1969 Apr;16(4):485–491. doi: 10.1111/j.1471-4159.1969.tb06847.x. [DOI] [PubMed] [Google Scholar]
  5. Kakiuchi S., Rall T. W. The influence of chemical agents on the accumulation of adenosine 3',5'-Phosphate in slices of rabbit cerebellum. Mol Pharmacol. 1968 Jul;4(4):367–378. [PubMed] [Google Scholar]
  6. Klein I., Levey G. S. Activation of myocardial adenyl cyclase by histamine in guinea pig, cat, and human heart. J Clin Invest. 1971 May;50(5):1012–1015. doi: 10.1172/JCI106571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miyamoto E., Kuo J. F., Greengard P. Adenosine 3',5'-monophosphate-dependent protein kinase from brain. Science. 1968 Jul 4;165(3888):63–65. [PubMed] [Google Scholar]
  8. Perkins J. P., Macintyre E. H., Riley W. D., Clark R. B. Adenyl cyclase, phosphodiesterase and cyclic AMP dependent protein kinase of malignant glial cells in culture. Life Sci I. 1971 Sep 15;10(18):1069–1080. doi: 10.1016/0024-3205(71)90181-0. [DOI] [PubMed] [Google Scholar]
  9. Pontén J., Macintyre E. H. Long term culture of normal and neoplastic human glia. Acta Pathol Microbiol Scand. 1968;74(4):465–486. doi: 10.1111/j.1699-0463.1968.tb03502.x. [DOI] [PubMed] [Google Scholar]
  10. Robison G. A., Butcher R. W., Sutherland E. W. Cyclic AMP. Annu Rev Biochem. 1968;37:149–174. doi: 10.1146/annurev.bi.37.070168.001053. [DOI] [PubMed] [Google Scholar]
  11. SUTHERLAND E. W., RALL T. W., MENON T. Adenyl cylase. I. Distribution, preparation, and properties. J Biol Chem. 1962 Apr;237:1220–1227. [PubMed] [Google Scholar]
  12. Sattin A., Rall T. W. The effect of adenosine and adenine nucleotides on the cyclic adenosine 3', 5'-phosphate content of guinea pig cerebral cortex slices. Mol Pharmacol. 1970 Jan;6(1):13–23. [PubMed] [Google Scholar]
  13. Shimizu H., Creveling C. R., Daly J. Stimulated formation of adenosine 3',5'-cyclic phosphate in cerebral cortex: synergism between electrical activity and biogenic amines. Proc Natl Acad Sci U S A. 1970 Apr;65(4):1033–1040. doi: 10.1073/pnas.65.4.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES