Abstract
Enveloped particles of herpes simplex virus produced in human cells in culture contained spermidine and spermine, in a molar ratio of 1.6 ± 0.2. The spermine present within the nucleocapsid is sufficient to neutralize at least 40% of the viral DNA. Disruption of the envelope with nonionic detergent and urea resulted in the selective loss of spermidine. Exogenous ornithine can function as a precursor to host and viral polyamines only before infection.
Keywords: nucleocapsid, viral envelope, viral core, DNA phosphate-group neutralization, human carcinoma cells
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Selected References
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