Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Nov;68(11):2822–2825. doi: 10.1073/pnas.68.11.2822

Association Factor of Ribosomal Subunits from Bacillus stearothermophilus

M García-Patrone 1,2, N S González 1,2, I D Algranati 1,2
PMCID: PMC389534  PMID: 4941986

Abstract

The isolation of a new factor, which can cause the in vitro association of 30S and 50S ribosomal subunits at low Mg++ concentration, is described. The association factor is eluted together with the dissociation protein when ribosomes of Bacillus stearothermophilus are washed with salt solutions of high concentration. The association activity is heat-stable, whereas dissociation factor is inactivated after 10 min at 80°C. This treatment allows the separation of both factors. Several properties rule out the possibility that uncharged, amino-acyl-, or peptidyl-tRNA are responsible for the association process described in this report. Digestion with trypsin shows that the association factor contains at least two components, one of which is a protein.

Keywords: ribosomal cycle, E. coli, dissociation factor, heat inactivation

Full text

PDF
2822

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Algranati I. D., Gonzalez N. S., Bade E. G. Physiological role of 70S ribosomes in bacteria. Proc Natl Acad Sci U S A. 1969 Feb;62(2):574–580. doi: 10.1073/pnas.62.2.574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Algranati I. D. Occurrence of termination ribosomes as free 70 S particles. FEBS Lett. 1970 Oct 5;10(3):153–155. doi: 10.1016/0014-5793(70)80440-9. [DOI] [PubMed] [Google Scholar]
  3. Bade E. G., González N. S., Algranati I. S. Dissociation of 70S ribosomes: some properties of the dissociating factor from Bacillus stearothermophilus and Escherichia coli. Proc Natl Acad Sci U S A. 1969 Oct;64(2):654–660. doi: 10.1073/pnas.64.2.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Belitsina N. V., Spirin A. S. Studies on the structure of ribosomes. IV. Participation of aminoacyl-transfer RNA and peptidyl-transfer RNA in the association of ribosomal subparticles. J Mol Biol. 1970 Aug 28;52(1):45–55. doi: 10.1016/0022-2836(70)90176-2. [DOI] [PubMed] [Google Scholar]
  5. Davis B. D. Role of subunits in the ribosome cycle. Nature. 1971 May 21;231(5299):153–157. doi: 10.1038/231153a0. [DOI] [PubMed] [Google Scholar]
  6. Dubnoff J. S., Maitra U. Isolation and properties of polypeptide chain initiation factor FII from Escherichia coli: evidence for a dual function. Proc Natl Acad Sci U S A. 1971 Feb;68(2):318–323. doi: 10.1073/pnas.68.2.318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. García-Patrone M., Perazzolo C. A., Baralle F., González N. S., Algranati I. D. Studies on dissociation factor of bacterial ribosomes: effect of antibiotics. Biochim Biophys Acta. 1971 Aug 26;246(2):291–299. doi: 10.1016/0005-2787(71)90139-0. [DOI] [PubMed] [Google Scholar]
  8. Ghysen A., Bollen A., Herzog A. Ionic effects on the ribosomal quaternary structure. Eur J Biochem. 1970 Mar 1;13(1):132–136. doi: 10.1111/j.1432-1033.1970.tb00908.x. [DOI] [PubMed] [Google Scholar]
  9. Gonzalez N. S., Bade E. G., Algranati I. D. Effect of GTP on the dissociation of 70 S ribosomes. FEBS Lett. 1969 Aug;4(4):331–334. doi: 10.1016/0014-5793(69)80268-1. [DOI] [PubMed] [Google Scholar]
  10. Guthrie C., Nomura M. Initiation of protein synthesis: a critical test of the 30S subunit model. Nature. 1968 Jul 20;219(5151):232–235. doi: 10.1038/219232a0. [DOI] [PubMed] [Google Scholar]
  11. Hardy S. J., Turnock G. Stabilization of 70S ribosomes by spermidine. Nat New Biol. 1971 Jan 6;229(1):17–19. doi: 10.1038/newbio229017a0. [DOI] [PubMed] [Google Scholar]
  12. J Talens, Kalousek F., Bosch L. Dissociation of 70 S E. coli ribosomes induced by a ribosomal factor (DF). Electrophoretic studies of the ribosomal particles. FEBS Lett. 1970 Dec 23;12(1):4–8. doi: 10.1016/0014-5793(70)80580-4. [DOI] [PubMed] [Google Scholar]
  13. Kaempfer R. O., Meselson M., Raskas H. J. Cyclic dissociation into stable subunits and re-formation of ribosomes during bacterial growth. J Mol Biol. 1968 Jan 28;31(2):277–289. doi: 10.1016/0022-2836(68)90444-0. [DOI] [PubMed] [Google Scholar]
  14. Kaempfer R. Dissociation of ribosomes on polypeptide chain termination and origin of single ribosomes. Nature. 1970 Nov 7;228(5271):534–537. doi: 10.1038/228534a0. [DOI] [PubMed] [Google Scholar]
  15. Kaempfer R. Ribosomal subunit exchange during protein synthesis. Proc Natl Acad Sci U S A. 1968 Sep;61(1):106–113. doi: 10.1073/pnas.61.1.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Morimoto T. Intermediate stage in the association and dissociation of Escherichia coli ribosomes and the combining properties of their subunits. Biochim Biophys Acta. 1969 May 20;182(1):135–146. doi: 10.1016/0005-2787(69)90528-0. [DOI] [PubMed] [Google Scholar]
  17. Sabol S., Sillero M. A., Iwasaki K., Ochoa S. Purification and properties of initiation factor F3. Nature. 1970 Dec 26;228(5278):1269–1273. doi: 10.1038/2281269a0. [DOI] [PubMed] [Google Scholar]
  18. Schlessinger D., Mangiarotti G., Apirion D. The formation and stabilization of 30S and 50S ribosome couples in Escherichia coli. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1782–1789. doi: 10.1073/pnas.58.4.1782. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schreier M. H., Noll H. Chain initiation in primitive protein synthesis: a 60S intermediate in the formation of active 70S ribosomes. Nature. 1970 Jul 11;227(5254):128–133. doi: 10.1038/227128a0. [DOI] [PubMed] [Google Scholar]
  20. Schreier M. H., Noll H. Conformational changes in ribosomes during protein synthesis. Proc Natl Acad Sci U S A. 1971 Apr;68(4):805–809. doi: 10.1073/pnas.68.4.805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Spirin A. S. A model of the functioning ribosome: locking and unlocking of the ribosome subparticles. Cold Spring Harb Symp Quant Biol. 1969;34:197–207. doi: 10.1101/sqb.1969.034.01.026. [DOI] [PubMed] [Google Scholar]
  22. Subramanian A. R., Davis B. D., Beller R. J. The ribosome dissociation factor and the ribosome-polysome cycle. Cold Spring Harb Symp Quant Biol. 1969;34:223–230. doi: 10.1101/sqb.1969.034.01.028. [DOI] [PubMed] [Google Scholar]
  23. Subramanian A. R., Ron E. Z., Davis B. D. A factor required for ribosome dissociation in Escherichia coli. Proc Natl Acad Sci U S A. 1968 Oct;61(2):761–767. doi: 10.1073/pnas.61.2.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tamaoki T., Miyazawa F. Dissociation of ribosomes at high temperatures. J Mol Biol. 1966 Jun;17(2):537–540. doi: 10.1016/s0022-2836(66)80164-x. [DOI] [PubMed] [Google Scholar]
  25. van Diggelen O. P., Heinsius H. L., Kalousek F., Bosh L. Formation of 61 s and 70 s particles from ribosomal subunits of Escherichia coli. J Mol Biol. 1971 Jan 28;55(2):277–281. doi: 10.1016/0022-2836(71)90198-7. [DOI] [PubMed] [Google Scholar]
  26. van Duin J., VAN Dieijen G., Dieijen G., van Knippenberg P. H., Bosch L. Different species of 70S ribosomes of Escherichia coli and their dissociation into subunits. Eur J Biochem. 1970 Dec;17(3):433–440. doi: 10.1111/j.1432-1033.1970.tb01183.x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES