NG2 accelerates the rate of plasmin generation. (A) tPA-catalyzed plasmin generation was measured using an amidolytic assay. Increasing molar concentrations of NG2 (■), NG2 pretreated with Chondroitinase (▼), or fibrin (○) were incubated with tPA, plg, and amiloride, followed by the addition of the chromogenic substrate S-2251. (B) NG2 was incubated with tPA (0.028 μM), and plg and plasmin generation was quantified. Additional tPA (0.28 μM) was added to some samples as shown. Error bars represent S.E.M where #P < 0.05 and **P < 0.01 by ANOVA followed by Bonferroni’s post hoc test.