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. Author manuscript; available in PMC: 2014 Jan 19.
Published in final edited form as: Glia. 2008 Jan 15;56(2):177–189. doi: 10.1002/glia.20603

Fig. 6.

Fig. 6

In vitro and ex vivo degradation of NG2 by plasmin. (A) Plasmin [generated by tPA’s (20 ng) action on plg (400 ng)] was incubated with recombinant NG2, as indicated. Degradation products were visualized by silver staining. NG2 runs at molecular weights of 250 and 148 kDa (arrows). NG2 degradation by plasmin was quantified by densitometry and plotted as mean intensity. MW: molecular weight markers in the first lane. This experiment was repeated three times. (B) Injured spinal cord homogenates (in two separate experiments) were incubated with tPA (20 ng) and plg (100 ng) and analyzed by SDS-PAGE. NG2 was detected by western blot analysis using a mouse anti-NG2 antibody. Amounts of the remaining full-length NG2 at the different time points were quantified by densitometry and plotted as mean intensity.