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. 1971 Dec;68(12):2954–2957. doi: 10.1073/pnas.68.12.2954

Slow Joining of Newly Replicated DNA Chains in DNA Polymerase I-Deficient Escherichia coli Mutants*

Reiji Okazaki 1, Mikio Arisawa 1, Akio Sugino 1
PMCID: PMC389568  PMID: 4943548

Abstract

In Escherichia coli mutants deficient in DNA polymerase I, newly replicated short DNA is joined at about 10% of the rate in the wild-type strains. It is postulated that DNA polymerase I normally functions in filling gaps between the nascent short segments synthesized by the replication complex. Possible implications of the finding are discussed in relation to other abnormal properties of these mutants.

Keywords: DNA biosynthesis, discontinuous replication, pulse labeling, density gradient centrifugation, DNA polymerase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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