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. 1971 Dec;68(12):2967–2971. doi: 10.1073/pnas.68.12.2967

In Vitro Excision-Repair of Ultraviolet-Irradiated Transforming DNA from Bacillus subtilis

H L Heijneker †,*, H Pannekoek †, R A Oosterbaan †, P H Pouwels †, S Bron ‡, F Arwert ‡, G Venema ‡
PMCID: PMC389571  PMID: 5002281

Abstract

The biological activity of UV-inactivated Bacillus subtilis DNA is partly restored after incubation with a UV-specific endonuclease from Micrococcus lutens in conjunction with DNA polymerase and DNA ligase, both isolated from Escherichia coli. The restored activity is not further increased by photoreactivation. Pyrimidine dimers are specifically liberated when irradiated DNA is exposed to the three enzymes. None of these effects is observed when pancreatic DNase is used instead of UV-specific endonuclease.

Keywords: DNA excision, DNA ligase, gene repair, F-strain, DNA polymerase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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