Abstract
The three spectroscopically distinct classes of phycobiliproteins characteristic of the Cyanophyta and Rhodophyta—phycocyanins, allophycocyanins, and phycoerythrins—share no common antigenic determinants detectable by the Ouchterlony double diffusion technique. Each class of phycobiliprotein, from both Cyanophyta and Rhodophyta, possesses a strong determinant common to all members of that class. With respect to an antiserum directed against a specific cyanophytan biliprotein, all heterologous biliproteins of the same class are immunologically identical, as shown by the fact that absorption with a given heterologous antigen simultaneously eliminates crossreactions with other heterologous antigens. A cryptophytan phycoerythrin was found to be immunologically unrelated to any of the cyanophytan or rhodophytan biliproteins examined.
Keywords: Ouchterlony double diffusion, Cyanophyta, Rhodophyta, phycobiliproteins
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bennett A., Bogorad L. Properties of subunits and aggregates of blue-green algal biliproteins. Biochemistry. 1971 Sep 14;10(19):3625–3634. doi: 10.1021/bi00795a022. [DOI] [PubMed] [Google Scholar]
- Berns D. S. Immunochemistry of biliproteins. Plant Physiol. 1967 Nov;42(11):1569–1586. doi: 10.1104/pp.42.11.1569. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chapman D. J., Cole W. J., Siegelman H. W. Chromophores of allophycocyanin and R-phycocyanin. Biochem J. 1967 Dec;105(3):903–905. doi: 10.1042/bj1050903. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glazer A. N., Cohen-Bazire G. Subunit structure of the phycobiliproteins of blue-green algae. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1398–1401. doi: 10.1073/pnas.68.7.1398. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schram B. L., Kroes H. H. Structure of phycocyanobilin. Eur J Biochem. 1971 Apr 30;19(4):581–594. doi: 10.1111/j.1432-1033.1971.tb01352.x. [DOI] [PubMed] [Google Scholar]
- Siegelman H. W., Turner B. C., Hendricks S. B. The chromophore of phytochrome. Plant Physiol. 1966 Oct;41(8):1289–1292. doi: 10.1104/pp.41.8.1289. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stanier R. Y., Kunisawa R., Mandel M., Cohen-Bazire G. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev. 1971 Jun;35(2):171–205. doi: 10.1128/br.35.2.171-205.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]