Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Dec;68(12):3049–3053. doi: 10.1073/pnas.68.12.3049

Structure of the Replicating DNA from Bacteriophage T4*

Hajo Delius †, Chin Howe ‡, Andrzej W Kozinski ‡
PMCID: PMC389588  PMID: 4943552

Abstract

At an early stage of replication, parental T4 DNA shows a loop structure often displaying two 3′-ended, single-stranded “whiskers”, located in trans configuration at the branching-points. Several such loops have been observed within a single T4 molecule. Occasionally, reinitiation occurred in the middle of a loop, which suggests that the loop was growing in both directions.

Keywords: E. coli, electron microscopy, loops, whiskers, initiation

Full text

PDF
3049

Images in this article

3050Image
on p.3050
3051Image
on p.3051

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberts B. M., Amodio F. J., Jenkins M., Gutmann E. D., Ferris F. L. Studies with DNA-cellulose chromatography. I. DNA-binding proteins from Escherichia coli. Cold Spring Harb Symp Quant Biol. 1968;33:289–305. doi: 10.1101/sqb.1968.033.01.033. [DOI] [PubMed] [Google Scholar]
  2. CAIRNS J. The bacterial chromosome and its manner of replication as seen by autoradiography. J Mol Biol. 1963 Mar;6:208–213. doi: 10.1016/s0022-2836(63)80070-4. [DOI] [PubMed] [Google Scholar]
  3. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  4. Kozinski A. W., Kozinski P. B. Early intracellular events in the replication T4 phage DNA. II. Partially replicated DNA. Proc Natl Acad Sci U S A. 1965 Aug;54(2):634–640. doi: 10.1073/pnas.54.2.634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kozinski A. W., Kozinski P. B., James R. Molecular recombination in T4 bacteriophage deoxyribonucleic acid. I. Tertiary structure of early replicative and recombining deoxyribonucleic acid. J Virol. 1967 Aug;1(4):758–770. doi: 10.1128/jvi.1.4.758-770.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kozinski A. W., Lin T. H. Early intracellular events in the replication of T4 phage DNA. I. Complex formation of replicative DNA. Proc Natl Acad Sci U S A. 1965 Jul;54(1):273–278. doi: 10.1073/pnas.54.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lee C. S., Davis R. W., Davidson N. A physical study by electron microscopy of the terminally reptitious, circularly permuted DNA from the coliphage particles of Escherichia coli 15. J Mol Biol. 1970 Feb 28;48(1):1–22. doi: 10.1016/0022-2836(70)90215-9. [DOI] [PubMed] [Google Scholar]
  8. Miller R. C., Jr, Kozinski A. W. Early intracellular events in the replication of bacteriophage T4 deoxyribonucleic acid. V. Further studies on the T4 protein-deoxyribonucleic acid complex. J Virol. 1970 Apr;5(4):490–501. doi: 10.1128/jvi.5.4.490-501.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mosig G. A preferred origin and direction of bacteriophage T4 DNA replication. I. A gradient of allele frequencies in crosses between normal and small T4 particles. J Mol Biol. 1970 Nov 14;53(3):503–514. doi: 10.1016/0022-2836(70)90080-x. [DOI] [PubMed] [Google Scholar]
  10. Schnös M., Inman R. B. Position of branch points in replicating lambda DNA. J Mol Biol. 1970 Jul 14;51(1):61–73. doi: 10.1016/0022-2836(70)90270-6. [DOI] [PubMed] [Google Scholar]
  11. Werner R. Distribution of growing points in DNa of bacteriophage T4. J Mol Biol. 1968 May 14;33(3):679–692. doi: 10.1016/0022-2836(68)90313-6. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES