Table 1.
Representative 5‘->3‘ sequences after 7 rounds of SELMA selection, Kd for binding to 2G12, and consensus sequence.
| Clone ID | Sequence ( = glycosylated EdU, yellow = consensus, blue=constant primer regions) |
Kd | Bmax |
|---|---|---|---|
| 7A8-4 |  |
> 1 µM | NDa |
| 7A8-9 |  |
> 1 µM | NDa |
| 15A8-B |  |
> 1 µM | NDa |
| 15A8-F |  |
410±90 | 0.85±0.10 |
| M3-HH |  |
320±80 | 0.65±0.08 |
| 7A8-1 |  |
310±110 | 0.73±0.13 |
| 7A8-8 |  |
320±10 | 0.86±0.02 |
| 15A8-J |  |
n.b.b | 0 |
| M3-30 |  |
n.b.b | 0 |
| F1 controlc |  |
430±130 | 0.33±0.06 |
a
Not determined; binding curve was linear up to highest 2G12 concentration tested.
b
no binding.
c
10-Man4 winner from previously published selection with 25 % glycosylated library (Ref 4).