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. 1971 Dec;68(12):3154–3157. doi: 10.1073/pnas.68.12.3154

Stimulation of Glucose Transport in Cultures of Density-Inhibited Chick Embryo Cells

Bartholomew M Sefton 1,2,*, Harry Rubin 1,2,†
PMCID: PMC389611  PMID: 5289253

Abstract

The rate of glucose transport in sparse, rapidly growing chick-embryo fibroblasts is much greater than that in density-inhibited cells. The addition of fresh chicken serum or trypsin to the medium of density-inhibited cells causes a large increase in the rate of glucose transport that is detectable 15 min after addition. The increase in glucose transport precedes the increase in DNA synthesis by 5-6 hr. Only small changes in rates of transport are seen with nucleosides or a nonmetabolizable amino acid. The increase in glucose transport requires protein synthesis but not RNA or DNA synthesis.

Keywords: serum, trypsin, cycloheximide, actinomycin D, 2-deoxyglucose

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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