Abstract
Evidence is presented that suggests that GTP (or 5′-guanylylmethylene diphosphonate) is not essential for either formation of an AUG-directed, initiation factor IF-2-dependent initiation complex on the 30S subunit or for positioning of fMet-tRNA on the peptidyl site. However, recycling of limiting amounts of IF-2 requires both GTP and the 50S subunit. The formation of the complex IF-2-30S as an intermediate in polypeptidechain initiation is suggested.
Keywords: 30S-initiation complex, fMet-puromycin, recycling of IF-2, 50S subunit, IF-1
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Selected References
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