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. 1972 Oct;69(10):2793–2797. doi: 10.1073/pnas.69.10.2793

An Osmotically Fragile Mutant of Bacillus subtilis with an Active Membrane-Associated Phospholipase A1

Claudia Kent 1, W J Lennarz 1
PMCID: PMC389646  PMID: 4628095

Abstract

By use of a newly developed procedure for the isolation of mutants with osmotically fragile protoplasts, a mutant of Bacillus subtilis was isolated that has a very active enzyme system for the catabolism of phospholipids via the sequential action of a phospholipase A1 (EC 3.1.1.4) and a lysophospholipase (EC 3.1.1.5). The wild-type bacteria contain no detectable phospholipase A1 activity, but do contain a protein that specifically inhibits the phospholipase A1 in the mutant. This protein may play an important role in the control of phospholipid catabolism.

Keywords: protoplasts, lysophospholipase, phospholipase A1 inhibitor

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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