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. 1972 Oct;69(10):2798–2801. doi: 10.1073/pnas.69.10.2798

A Genetic Locus for Inducibility of C-Type Virus in BALB/c Cells: The Effect of a Nonlinked Regulatory Gene on Detection of Virus after Chemical Activation

John R Stephenson 1, Stuart A Aaronson 1
PMCID: PMC389647  PMID: 4344375

Abstract

Previous studies have shown that chemicals can transiently activate endogenous C-type viruses from embryo cells of the BALB/c mouse strain, but not from cells of another mouse strain, NIH Swiss. The number of genetic loci for inducibility of endogenous virus in BALB/c cells was investigated with cell lines derived from appropriate, F1, F2-hybrid, and backcross generation embryos of these strains. A single genetic locus responsible for inducibility of virus in BALB/c cells was detected, and tentatively designated Ind. A second locus, previously described in studies of mouse-cell susceptibility to exogenous virus infection, Fv-1, was found to be genetically nonlinked to Ind. This regulatory gene plays an important role in determining whether the induced viruses of BALB/c cells can persist after chemical activation.

Keywords: mouse embryo, endogenous virus, IdU

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Hartley J. W., Todaro G. J. Mouse leukemia virus: "spontaneous" release by mouse embryo cells after long-term in vitro cultivation. Proc Natl Acad Sci U S A. 1969 Sep;64(1):87–94. doi: 10.1073/pnas.64.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aaronson S. A., Todaro G. J., Scolnick E. M. Induction of murine C-type viruses from clonal lines of virus-free BALB-3T3 cells. Science. 1971 Oct 8;174(4005):157–159. doi: 10.1126/science.174.4005.157. [DOI] [PubMed] [Google Scholar]
  3. Axelrad A. Genetic control of susceptibility to Friend leukemia virus in mice: studies with the spleen focus assay method. Natl Cancer Inst Monogr. 1966 Sep;22:619–629. [PubMed] [Google Scholar]
  4. Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
  5. Hartley J. W., Rowe W. P., Capps W. I., Huebner R. J. Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture. J Virol. 1969 Feb;3(2):126–132. doi: 10.1128/jvi.3.2.126-132.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hartley J. W., Rowe W. P., Huebner R. J. Host-range restrictions of murine leukemia viruses in mouse embryo cell cultures. J Virol. 1970 Feb;5(2):221–225. doi: 10.1128/jvi.5.2.221-225.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Huebner R. J., Kelloff G. J., Sarma P. S., Lane W. T., Turner H. C., Gilden R. V., Oroszlan S., Meier H., Myers D. D., Peters R. L. Group-specific antigen expression during embryogenesis of the genome of the C-type RNA tumor virus: implications for ontogenesis and oncogenesis. Proc Natl Acad Sci U S A. 1970 Sep;67(1):366–376. doi: 10.1073/pnas.67.1.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jainchill J. L., Aaronson S. A., Todaro G. J. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. J Virol. 1969 Nov;4(5):549–553. doi: 10.1128/jvi.4.5.549-553.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lilly F. Susceptibility to two strains of Friend leukemia virus in mice. Science. 1967 Jan 27;155(3761):461–462. doi: 10.1126/science.155.3761.461. [DOI] [PubMed] [Google Scholar]
  10. Lowy D. R., Rowe W. P., Teich N., Hartley J. W. Murine leukemia virus: high-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine. Science. 1971 Oct 8;174(4005):155–156. doi: 10.1126/science.174.4005.155. [DOI] [PubMed] [Google Scholar]
  11. Pincus T., Hartley J. W., Rowe W. P. A major genetic locus affecting resistance to infection with murine leukemia viruses. I. Tissue culture studies of naturally occurring viruses. J Exp Med. 1971 Jun 1;133(6):1219–1233. doi: 10.1084/jem.133.6.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rowe W. P., Hartley J. W., Lander M. R., Pugh W. E., Teich N. Noninfectious AKR mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia virus. Virology. 1971 Dec;46(3):866–876. doi: 10.1016/0042-6822(71)90087-0. [DOI] [PubMed] [Google Scholar]
  13. Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
  14. Stephenson J. R., Aaronson S. A. Genetic factors influencing C-type RNA virus induction. J Exp Med. 1972 Jul 1;136(1):175–184. doi: 10.1084/jem.136.1.175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]

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