Figure 8. Glycosylation symmetry has a greater influence on ADCC than does afucosylation symmetry. In this figure we compare four distinct variants of carbohydrate composition, all of which are achieved by using heterodimer assembly technology described herein. The ADCC activities have been measured using an engineered NK cell line (assayed twice on two different days) and have been reported as relative values; normalized to WT. Shown here is the ability to tune ADCC activity with asymmetric carbohydrate composition. The graph suggests that though afucosylation of a hemi-glycosylated antibody influences ADCC activity, symmetry of glycosylation garners a more significant impact.