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. 1972 Oct;69(10):2879–2884. doi: 10.1073/pnas.69.10.2879

DNA-Dependent DNA Polymerases I and II from Normal Human-Blood Lymphocytes

R Graham Smith 1, Robert C Gallo 1
PMCID: PMC389666  PMID: 4342965

Abstract

Two DNA polymerases have been purified from blood lymphocytes of normal human individuals. In contrast to a DNA polymerase isolated from lymphoblasts from individuals with leukemia, neither of the DNA polymerases from normal lymphocytes transcribes the heteropolymeric regions of tumor-virus RNA into DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOLLUM F. J. Calf thymus polymerase. J Biol Chem. 1960 Aug;235:2399–2403. [PubMed] [Google Scholar]
  2. Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
  3. Baltimore D., Smoler D. Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1507–1511. doi: 10.1073/pnas.68.7.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baril E. F., Brown O. E., Jenkins M. D., Laszlo J. Deoxyribonucleic acid polymerase with rat liver ribosomes and smooth membranes. Purification and properties of the enzymes. Biochemistry. 1971 May 25;10(11):1981–1992. doi: 10.1021/bi00787a004. [DOI] [PubMed] [Google Scholar]
  5. Chang L. M., Bollum F. J. Antigenic relationships in mammalian DNA polymerase. Science. 1972 Mar 10;175(4026):1116–1117. doi: 10.1126/science.175.4026.1116. [DOI] [PubMed] [Google Scholar]
  6. Chang L. M., Bollum F. J. Low molecular weight deoxyribonucleic acid polymerase from rabbit bone marrow. Biochemistry. 1972 Mar 28;11(7):1264–1272. doi: 10.1021/bi00757a023. [DOI] [PubMed] [Google Scholar]
  7. Chang L. M., Bollum F. J. Low molecular weight deoxyribonucleic acid polymerase in mammalian cells. J Biol Chem. 1971 Sep 25;246(18):5835–5837. [PubMed] [Google Scholar]
  8. Darnell J. E., Philipson L., Wall R., Adesnik M. Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA. Science. 1971 Oct 29;174(4008):507–510. doi: 10.1126/science.174.4008.507. [DOI] [PubMed] [Google Scholar]
  9. Furth J. J., Cohen S. S. Inhibition of mammalian DNA polymerase by the 5'-triphosphate of 1-beta-d-arabinofuranosylcytosine and the 5'-triphosphate of 9-beta-d-arabinofuranoxyladenine. Cancer Res. 1968 Oct;28(10):2061–2067. [PubMed] [Google Scholar]
  10. Gallo R. C., Yang S. S., Ting R. C. RNA dependent DNA polymerase of human acute leukaemic cells. Nature. 1970 Dec 5;228(5275):927–929. doi: 10.1038/228927a0. [DOI] [PubMed] [Google Scholar]
  11. Goodman N. C., Spiegelman S. Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2203–2206. doi: 10.1073/pnas.68.9.2203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Greene R., Korn D. Partial purification and characterization of deoxyribonucleic acid polymerase from KB cells. J Biol Chem. 1970 Jan 25;245(2):254–261. [PubMed] [Google Scholar]
  13. Hanafusa H., Hanafusa T. Noninfectious RSV deficient in DNA polymerase. Virology. 1971 Jan;43(1):313–316. doi: 10.1016/0042-6822(71)90251-0. [DOI] [PubMed] [Google Scholar]
  14. Lai M. M., Duesberg P. H. Adenylic acid-rich sequence in RNAs of Rous sarcoma virus and Rauscher mouse leukaemia virus. Nature. 1972 Feb 18;235(5338):383–386. doi: 10.1038/235383c0. [DOI] [PubMed] [Google Scholar]
  15. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  16. McEwen C. R. Tables for estimating sedimentation through linear concentration gradients of sucrose solution. Anal Biochem. 1967 Jul;20(1):114–149. doi: 10.1016/0003-2697(67)90271-0. [DOI] [PubMed] [Google Scholar]
  17. Miller J. A., Miller E. C. Chemical carcinogenesis: mechanisms and approaches to its control. J Natl Cancer Inst. 1971 Sep;47(3):V–XIV. [PubMed] [Google Scholar]
  18. Moses R. E., Richardson C. C. A new DNA polymerase activity of Escherichia coli. I. Purification and properties of the activity present in E. coli polA1. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1557–1564. doi: 10.1016/0006-291x(70)90565-6. [DOI] [PubMed] [Google Scholar]
  19. Müller W. E., Yamazaki Z. I., Zahn R. K., Brehm G., Korting G. RNA dependent DNA polymerase in cells of xeroderma pigmentosum. Biochem Biophys Res Commun. 1971 Jul 16;44(2):433–438. doi: 10.1016/0006-291x(71)90619-x. [DOI] [PubMed] [Google Scholar]
  20. Penner P. E., Cohen L. H., Loeb L. A. RNA-dependent DNA polymerase in human lymphocytes during gene activation by phytohaemagglutinin. Nat New Biol. 1971 Jul 14;232(28):58–61. doi: 10.1038/newbio232058a0. [DOI] [PubMed] [Google Scholar]
  21. Robert M. S., Smith R. G., Gallo R. C., Sarin P. S., Abrell J. W. Viral and cellular DNA polymerase: comparison of activities with synthetic and natural RNA templates. Science. 1972 May 19;176(4036):798–800. doi: 10.1126/science.176.4036.798. [DOI] [PubMed] [Google Scholar]
  22. Schlabach A., Fridlender B., Bolden A., Weissbach A. DNA-dependent DNA polymerases from HeLa cell nuclei. II. Template and substrate utilization. Biochem Biophys Res Commun. 1971 Aug 20;44(4):879–885. doi: 10.1016/0006-291x(71)90793-5. [DOI] [PubMed] [Google Scholar]
  23. Scolnick E. M., Aaronson S. A., Todaro G. J., Parks W. P. RNA dependent DNA polymerase activity in mammalian cells. Nature. 1971 Jan 29;229(5283):318–321. doi: 10.1038/229318a0. [DOI] [PubMed] [Google Scholar]
  24. Slater I., Slater D. W. DNA polymerase potentials of sea urchin embryos. Nat New Biol. 1972 May 17;237(72):81–85. doi: 10.1038/newbio237081a0. [DOI] [PubMed] [Google Scholar]
  25. Stavrianopoulos J. G., Karkas J. D., Chargaff E. Nucleic acid polymerases of the developing chicken embryo: a DNA polymerase preferring a hybrid template. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2207–2211. doi: 10.1073/pnas.68.9.2207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Temin H. M. Mechanism of cell transformation by RNA tumor viruses. Annu Rev Microbiol. 1971;25:609–648. doi: 10.1146/annurev.mi.25.100171.003141. [DOI] [PubMed] [Google Scholar]
  27. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]
  28. Ting R. C., Yang S. S., Gallo R. C. Reverse transcriptase, RNA tumour virus transformation and derivatives of rifamycin SV. Nat New Biol. 1972 Apr 12;236(67):163–166. doi: 10.1038/newbio236163a0. [DOI] [PubMed] [Google Scholar]
  29. Weissbach A., Schlabach A., Fridlender B., Bolden A. DNA polymerases from human cells. Nat New Biol. 1971 Jun 9;231(23):167–170. doi: 10.1038/newbio231167a0. [DOI] [PubMed] [Google Scholar]
  30. Wells R. D., Flügel R. M., Larson J. E., Schendel P. F., Sweet R. W. Comparison of some reactions catalyzed by deoxyribonucleic acid polymerase from avian myeloblastosis virus, Escherichia coli, and Micrococcus luteus. Biochemistry. 1972 Feb 15;11(4):621–629. doi: 10.1021/bi00754a025. [DOI] [PubMed] [Google Scholar]

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