Abstract
When a thermosensitive mutant of E. coli affected in DNA initiation is heated to 41°, a protein needed for DNA initiation is irreversibly denatured. When these bacteria are incubated at 30° in the presence of several concentrations of chloramphenicol, DNA synthesis is greatly stimulated at a chloramphenicol, DNA synthesis is greatly stimulated at a chloramphenicol concentration of 2.5 μg/ml, and then sharply decreases. This stimulation of DNA synthesis appears to be caused by an increased frequency of initiation. The existence of an “anti-initiator” protein controlling the frequency of chromosomal initiation is proposed.
Keywords: thermosensitive mutant, chloramphenicol, reinitiations, anti-initiator protein
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Selected References
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