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. 1972 Oct;69(10):2959–2962. doi: 10.1073/pnas.69.10.2959

Fusion of Mammalian Somatic Cells by Microsurgery

Elaine G Diacumakos 1, Edward L Tatum 1
PMCID: PMC389683  PMID: 4507616

Abstract

Mammalian somatic-cell hybrids can be formed in a direct and precise manner by microsurgery. Fusion of cells brought about in this way permits selection of specific, identifiable cells of the same or different types or species and their fusion at telophase.

Keywords: hybridization, HeLa, fibroblasts

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Croce C. M., Koprowski H., Eagle H. Effect of environmental pH on the efficiency of cellular hybridization. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1953–1956. doi: 10.1073/pnas.69.7.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Croce C. M., Sawicki W., Kritchevsky D., Koprowski H. Induction of homokaryocyte, heterokaryocyte and hybrid formation by lysolecithin. Exp Cell Res. 1971 Aug;67(2):427–435. doi: 10.1016/0014-4827(71)90428-9. [DOI] [PubMed] [Google Scholar]
  3. Diacumakos E. G., Holland S., Pecora P. A microsurgical methodology for human cells in vitro: evolution and applications. Proc Natl Acad Sci U S A. 1970 Apr;65(4):911–918. doi: 10.1073/pnas.65.4.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Diacumakos E. G., Holland S., Pecora P. Chromosome displacement in and extraction from human cells at different mitotic stages. Nature. 1971 Jul 2;232(5305):33–36. doi: 10.1038/232033a0. [DOI] [PubMed] [Google Scholar]
  5. Diacumakos E. G., Holland S., Pecora P. Microsurgical studies on human cells and cloning of HeLa cells. Nature. 1971 Jul 2;232(5305):28–32. doi: 10.1038/232028a0. [DOI] [PubMed] [Google Scholar]
  6. Lucy J. A., Ahkong Q. F., Cramp F. C., Fisher D., Howell J. I. Cell fusion without viruses. Biochem J. 1971 Oct;124(5):46P–47P. doi: 10.1042/bj1240046p. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Neff J. M., Enders J. F. Poliovirus replication and cytopathogenicity in monolayer hamster cell cultures fused with beta propiolactone-inactivated Sendai virus. Proc Soc Exp Biol Med. 1968 Jan;127(1):260–267. doi: 10.3181/00379727-127-32668. [DOI] [PubMed] [Google Scholar]
  8. Okada Y., Murayama F. Fusion of cells by HVJ: requirement of concentration of virus particles at the site of contact of two cells for fusion. Exp Cell Res. 1968 Sep;52(1):34–42. doi: 10.1016/0014-4827(68)90545-4. [DOI] [PubMed] [Google Scholar]

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