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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Nov;69(11):3105–3109. doi: 10.1073/pnas.69.11.3105

Temperature-Sensitive Mutants of Escherichia coli Requiring Saturated and Unsaturated Fatty Acids for Growth: Isolation and Properties

Mark E Harder *, Ifor R Beacham *, John E Cronan Jr , Kathryn Beacham *, Joy L Honegger *, David F Silbert *,
PMCID: PMC389714  PMID: 4564200

Abstract

A procedure is described for selection of temperature-sensitive mutants affecting fatty-acid synthesis based upon radiation suicide of wild-type organisms by tritiated acetate selectively incorporated into fatty acids. At 37°, two of the mutants extensively incorporate fatty-acid supplements provided in the medium, and grow for extended periods only when a trans-unsaturated or a combination of saturated and cis-unsaturated fatty acids is available. In vivo fatty-acid synthesis, measured by [14C]acetate incorporation, is temperature-sensitive in these strains relative to protein synthesis and other non-lipid macromolecular syntheses using acetate. The biochemical nature of these mutations has not been identified.

Keywords: radiation suicide, [3H]acetate, lipids

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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