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. 1972 Nov;69(11):3115–3118. doi: 10.1073/pnas.69.11.3115

Functional Modification of 16S Ribosomal RNA by Kethoxal

Harry F Noller 1, Jonathan B Chaires 1
PMCID: PMC389716  PMID: 4564202

Abstract

Kethoxal reacts with 30S ribosomal subunits to give totally inactive particles, as measured by in vitro protein synthesis. It is postulated that functional modification occurs at the binding site for transfer RNA since (a) loss of specific binding of transfer RNA, but not binding of messenger RNA, is simultaneous with loss of protein synthesis, and (b) loss of activity is inhibited by bound transfer RNA. By means of in vitro reconstitution and labeling techniques, it is found that loss of transfer RNA-binding activity is correlated with the modification of six or seven guanine residues in 16S RNA.

Keywords: 30S ribosomal subunits, tRNA binding site, guanine residues, reconstitution, protein synthesis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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