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. 1972 Nov;69(11):3138–3141. doi: 10.1073/pnas.69.11.3138

DNA Modification Methylase Activity of Escherichia coli Restriction Endonucleases K and P

Allan Haberman 1, Janet Heywood 1, Matthew Meselson 1
PMCID: PMC389721  PMID: 4564204

Abstract

The highly purified restriction endonucleases of E. coli K and coliphage P1 transfer methyl groups from S-adenosylmethionine to adenine residues of unmodified DNA. Incubation of unmodified DNA with endonucleases K or P and S-adenosylmethionine renders the DNA resistant to restriction. The enzymes, therefore, have both restriction endonuclease and modification methylase activities.

Keywords: S-adenosylmethionine, 6-methylaminopurine, multifunctional enzymes

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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