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. Author manuscript; available in PMC: 2014 Apr 30.
Published in final edited form as: Biochemistry. 2013 Apr 16;52(17):2874–2887. doi: 10.1021/bi400136u

Figure 8.

Figure 8

Turnover of WT RCN anSMEcpe with Kp18Thr or Kp18alloThr. A) Time-dependent formation of 5′-dA (closed triangles) and disappearance of Kp18Thr (closed squares). Reaction mixtures contained 100 μM anSMEcpe, 1 mM SAM, 0.5 mM Kp18Thr, and 3 mM DT. Vmax/[ET] values for 5’-dA formation and Kp18Thr disappearance are 0.29 min-1 and 0.11 min-1, respectively. B) Time-dependent formation of 5′-dA (closed triangles) and disappearance of Kp18alloThr (closed squares). Reaction mixtures contained 100 μM anSMEcpe, 1 mM SAM, 0.5 mM Kp18alloThr, and 3 mM DT. Vmax/[ET] values for 5’-dA formation and Kp18alloThr disappearance are 0.07 min-1 and 0.007 min-1, respectively.