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. 1972 Nov;69(11):3185–3188. doi: 10.1073/pnas.69.11.3185

Structure of the Oxidized Form of a Flavodoxin at 2.5-Å Resolution: Resolution of the Phase Ambiguity by Anomalous Scattering

K D Watenpaugh *, L C Sieker *, L H Jensen *, J Legall †,‡, M Dubourdieu †
PMCID: PMC389731  PMID: 4508313

Abstract

Flavodoxin from Desulfovibrio vulgaris crystallizes in the oxidized form as well-formed, tetragonal bipyramids, space group P43212, unit-cell parameters, a = b = 51.6 Å, c = 139.6 Å, 8 molecules per unit cell.

The structure has been determined at 2.5-Å resolution with phases based on a single isomorphous derivative. The phase ambiguity of a single derivative was resolved by use of anomalous scattering from the single-site Sm+3.

The molecule has a five-strand pleated sheet core with two long helices on either side of the sheet. The flavin mononucleotide lies mostly buried on one side of the molecule, but the methyl groups, one edge of the flavin, and part of the ribityl are exposed at the surface.

Keywords: protein structure, flavin mononucleotide, single isomorphous derivative, x-ray diffraction

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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