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. 1972 Nov;69(11):3207–3210. doi: 10.1073/pnas.69.11.3207

Membrane Site Modified on Induction of the Transformation of Lymphocytes by Periodate

Abraham Novogrodsky 1, Ephraim Katchalski 1
PMCID: PMC389737  PMID: 4343958

Abstract

Lymphocytes isolated from different sources undergo blastogenesis after mild treatment with periodate. Incubation of mouse spleen lymphocytes with neuraminidase or papain markedly reduces their response to periodate. Transformation induced by concanavalin A of the enzymically treated cells is not impaired. Blastogenesis induced by periodate is decreased when periodate-treated lymphocytes are reacted with borohydride, hydroxylamine, or semicarbazide. Treatment with these compounds does not affect transformation of cells by concanavalin A. It is suggested that different membrane sites are involved in lymphocyte transformation induced by periodate and by concanavalin A. The periodate target site seems to include a glycoprotein complex containing sialic acid that yields, on oxidation, an aldehyde moiety that is essential for transformation.

Keywords: mouse spleen, concanavalin A, neuraminidase, papain

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blumberg S., Schechter I., Berger A. The purification of papain by affinity chromatography. Eur J Biochem. 1970 Jul;15(1):97–102. doi: 10.1111/j.1432-1033.1970.tb00981.x. [DOI] [PubMed] [Google Scholar]
  2. Blumenfeld O. O., Gallop P. M., Liao T. H. Modification and introduction of a specific radioactive label into the erythrocyte membrane sialoglycoproteins. Biochem Biophys Res Commun. 1972 Jul 11;48(1):242–251. doi: 10.1016/0006-291x(72)90369-5. [DOI] [PubMed] [Google Scholar]
  3. Borberg H., Yesner I., Gesner B., Silber R. The effect of N acetyl C-galactosamine and other sugars on the mitogenic activity and attachment of PHA to tonsil cells. Blood. 1968 Jun;31(6):747–757. [PubMed] [Google Scholar]
  4. Codington J. F., Sanford B. H., Jeanloz R. W. Glycoprotein coat of the TA 3 cell. I. Removal of carbohydrate and protein material from viable cells. J Natl Cancer Inst. 1970 Oct;45(4):637–647. [PubMed] [Google Scholar]
  5. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Inbar M., Sachs L. Structural difference in sites on the surface membrane of normal and transformed cells. Nature. 1969 Aug 16;223(5207):710–712. doi: 10.1038/223710a0. [DOI] [PubMed] [Google Scholar]
  7. Mazzei D., Novi C., Bazzi C. Mitogenic action of papain. Lancet. 1966 Oct 8;2(7467):802–803. doi: 10.1016/s0140-6736(66)90406-5. [DOI] [PubMed] [Google Scholar]
  8. Novogrodsky A., Katchalski E. Effect of phytohemagglutinin and prostaglandins on cyclic AMP synthesis in rat lymph node lymphocytes. Biochim Biophys Acta. 1970 Aug 14;215(2):291–296. doi: 10.1016/0304-4165(70)90027-9. [DOI] [PubMed] [Google Scholar]
  9. Novogrodsky A., Katchalski E. Lymphocyte transformation induced by concanavalin A and its reversion by methyl-alpha-D-mannopyranoside. Biochim Biophys Acta. 1971 Jan 28;228(2):579–583. doi: 10.1016/0005-2787(71)90064-5. [DOI] [PubMed] [Google Scholar]
  10. Novogrodsky A., Katchlaski E. Induction of lymphocyte transformation by periodate. FEBS Lett. 1971 Jan 30;12(5):297–300. doi: 10.1016/0014-5793(71)80203-x. [DOI] [PubMed] [Google Scholar]
  11. Parker J. W., O'Brien R. L., Lukes R. J., Steiner J. TRransformation of human lymphocytes by sodium periodate. Lancet. 1972 Jan 8;1(7741):103–104. doi: 10.1016/s0140-6736(72)90115-8. [DOI] [PubMed] [Google Scholar]
  12. Powell A. E., Leon M. A. Reversible interaction of human lymphocytes with the mitogen concanavalin A. Exp Cell Res. 1970 Oct;62(2):315–325. doi: 10.1016/0014-4827(70)90560-4. [DOI] [PubMed] [Google Scholar]
  13. ROBBINS J. H. TISSUE CULTURE STUDIES OF THE HUMAN LYMPHOCYTE. Science. 1964 Dec 25;146(3652):1648–1654. doi: 10.1126/science.146.3652.1648. [DOI] [PubMed] [Google Scholar]
  14. Simmons R. L., Rios A., Ray P. K., Lundgren G. Effect of neuraminidase on growth of a 3-methylcholanthrene-induced fibrosarcoma in normal and immunosuppressed syngeneic mice. J Natl Cancer Inst. 1971 Nov;47(5):1087–1094. [PubMed] [Google Scholar]
  15. Van Lenten L., Ashwell G. Studies on the chemical and enzymatic modification of glycoproteins. A general method for the tritiation of sialic acid-containing glycoproteins. J Biol Chem. 1971 Mar 25;246(6):1889–1894. [PubMed] [Google Scholar]
  16. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
  17. WIGZELL H. QUANTITATIVE TITRATIONS OF MOUSE H-2 ANTIBODIES USING CR-51-LABELLED TARGET CELLS. Transplantation. 1965 May;3:423–431. doi: 10.1097/00007890-196505000-00011. [DOI] [PubMed] [Google Scholar]

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