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. 1972 Nov;69(11):3211–3214. doi: 10.1073/pnas.69.11.3211

In Vitro Repair of X-Irradiated DNA Extracted from Bacillus subtilis Deficient in Polymerase I

Philip J Laipis *, A T Ganesan †,‡
PMCID: PMC389738  PMID: 4628873

Abstract

DNA extracted from x-irradiated cells of a mutant of Bacillus subtilis deficient in DNA polymerase I has greatly reduced biological activity. This DNA, which has many single-strand nicks, can be repaired in vitro with purified DNA polymerase I and DNA ligase or with lysates of wild-type B. subtilis cells, which restore biological activity and increase the single-strand molecular weight. Lysates of polymerase I-deficient B. subtilis cells cannot repair such irradiated DNA until purified DNA polymerase I or lysate from wild-type cells is added to the deficient lysate.

Keywords: DNA ligase, gene repair, transformation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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