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. 1972 Nov;69(11):3233–3237. doi: 10.1073/pnas.69.11.3233

Conversion of ϕX174 and fd Single-Stranded DNA to Replicative Forms in Extracts of Escherichia coli

Reed B Wickner 1, Michel Wright 1, Sue Wickner 1, Jerard Hurwitz 1
PMCID: PMC389743  PMID: 4564209

Abstract

ϕX174 and M13 (fd) single-stranded circular DNAs are converted to their replicative forms by extracts of E. coli pol A1 cells. We find that the ϕX174 DNA-dependent reaction requires Mg++, ATP, and all four deoxynucleoside triphosphates, but not CTP, UTP, or GTP. This reaction also involves the products of the dnaC, dnaD, dnaE (DNA polymerase III), and dnaG genes, but not that of dnaF (ribonucleotide reductase). The in vitro conversion of fd single-stranded DNA to the replicative form requires all four ribonucleoside triphosphates, Mg++, and all four deoxynucleoside triphosphates. The reaction involves the product of gene dnaE but not those of genes dnaC, dnaD, dnaF, or dnaG. The reaction with fd DNA is inhibited by rifampicin or antibody to RNA polymerase, while the reaction with ϕX174 DNA is not affected by either. With the ϕX174 DNA-dependent reaction, activities have been detected that specifically complement extracts of dnaA, dnaB, dnaC, dnaD, or dnaG mutants.

Keywords: dnaC, dnaD, and dnaG gene products; DNA polymerase III

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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