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. 1972 Nov;69(11):3251–3255. doi: 10.1073/pnas.69.11.3251

Regulation of Lymphocyte Responses In Vitro: Potentiation and Inhibition of Rat Lymphocyte Responses to Antigen and Mitogens by Cytochalasin B*

Masaru Yoshinaga 1,†, Aiko Yoshinaga 1, Byron H Waksman 1,‡
PMCID: PMC389747  PMID: 4539012

Abstract

Cytochalasin B, at concentration between 0.02 and 0.2 μg/ml, was slightly stimulatory to lymph-node cells from normal rats and greatly potentiated their response to phytohemagglutinin and low concentrations of concanavalin A (mitogens for thymus-derived lymphocytes); it also potentiated the response of thymocytes to phytohemagglutinin. The response of lymphnode cells to lipopolysaccharide endotoxin (a mitogen for thymus-independent lymphocytes) was also enhanced, but only at concentrations in the usual inhibitory range, possibly by inhibition of a “suppressor T-cell” response. Sensitized lymphocytes responding to antigen were not stimulated at all, except at a very high cell density, where inhibition of a “suppressor cell” response was also considered likely. At concentrations of 5-10 μg/ml or higher, cytochalasin B inhibited all responses tested.

Keywords: blast transformation, T-cells, B-cells

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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