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. 1972 Nov;69(11):3256–3259. doi: 10.1073/pnas.69.11.3256

Ribosomal RNA Genes of Neurospora: Isolation and Characterization

Sisir K Chattopadhyay *, David E Kohne †, Sisr K Dutta ‡
PMCID: PMC389748  PMID: 4264050

Abstract

DNA sequences in Neurospora crassa that code for ribosomal RNA have been isolated in a highly purified state by DNA-RNA hybridization. About 100 separate ribosomal RNA genes exist in each nucleus. These genes are composed of repeated sequences that are present about 100 times per nucleus. The individual members of this group of DNA sequences are essentially identical.

Keywords: gene isolation, DNA-RNA hybridization, DNA reassociation kinetics

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avery R. J., Midgley J. E., Pigott G. H. An analysis of the ribosomal ribonucleic acids of Escherichia coli by hybridization techniques. Biochem J. 1969 Nov;115(3):395–403. doi: 10.1042/bj1150395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birnstiel M. L., Sells B. H., Purdom I. F. Kinetic complexity of RNA molecules. J Mol Biol. 1972 Jan 14;63(1):21–39. doi: 10.1016/0022-2836(72)90519-0. [DOI] [PubMed] [Google Scholar]
  3. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  4. Brown D. D., Dawid I. B. Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA. Science. 1968 Apr 19;160(3825):272–280. doi: 10.1126/science.160.3825.272. [DOI] [PubMed] [Google Scholar]
  5. Fellner P., Ehresmann C., Ebel J. P. Nucleotide sequences present within the 16S ribosomal RNA of Escherichia coli. Nature. 1970 Jan 3;225(5227):26–29. doi: 10.1038/225026a0. [DOI] [PubMed] [Google Scholar]
  6. Kohne D. E. Isolation and characterization of bacterial ribosomal RNA cistrons. Biophys J. 1968 Oct;8(10):1104–1118. doi: 10.1016/S0006-3495(68)86542-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Laird C. D., McConaughy B. L., McCarthy B. J. Rate of fixation of nucleotide substitutions in evolution. Nature. 1969 Oct 11;224(5215):149–154. doi: 10.1038/224149a0. [DOI] [PubMed] [Google Scholar]
  8. MCCONKEY E. H., HOPKINS J. W. THE RELATIONSHIP OF THE NUCLEOLUS TO THE SYNTHESIS OF RIBOSOMAL RNA IN HELA CELLS. Proc Natl Acad Sci U S A. 1964 Jun;51:1197–1204. doi: 10.1073/pnas.51.6.1197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nomura M. Bacterial ribosome. Bacteriol Rev. 1970 Sep;34(3):228–277. doi: 10.1128/br.34.3.228-277.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wood D. D., Luck D. J. Hybridization of mitochondrial ribosomal RNA. J Mol Biol. 1969 Apr;41(2):211–224. doi: 10.1016/0022-2836(69)90386-6. [DOI] [PubMed] [Google Scholar]

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