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. 1972 Nov;69(11):3317–3321. doi: 10.1073/pnas.69.11.3317

Translational Control of Hemoglobin Synthesis by an Initiation Factor Required for Recycling of Ribosomes and for their Binding to Messenger RNA

Raymond Kaempfer 1, Jennifer Kaufman 1
PMCID: PMC389762  PMID: 4508325

Abstract

The continued recycling of ribosomes during protein synthesis in rabbit reticulocyte lysates at 37° requires an initiation factor whose activity is rapidly lost in the absence of added heme. Partially purified factor (i) fully maintains the polysomes; (ii) inhibits the association of 40S and 60S ribosomal subunits into single ribosomes; (iii) promotes the quantitative entry of added 60S subunits into polysomes; (iv) allows the accumulation of ribosomal subunits, instead of single ribosomes, when initiation is blocked with aurin tricarboxylate; and (v) is absolutely required for the binding of globin messenger RNA to ribosomes.

These properties suggest that this mammalian initiation factor functions analogously to bacterial IF-3. In addition, the translational control of globin synthesis by heme is exerted, directly or indirectly, through this factor.

Keywords: IF-3, mammalian ribosome cycle, R17 RNA binding

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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