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. 1972 Nov;69(11):3423–3427. doi: 10.1073/pnas.69.11.3423

Transfection Mediated by Mycoplasmatales Viral DNA

Alan Liss 1,2, Jack Maniloff 1,2
PMCID: PMC389785  PMID: 4508332

Abstract

DNA isolated from Mycoplasmatales viruses MVL51 and MVGs51 was infectious when mixed with Acholeplasma laidlawii BN1-Na1R cells. Infectivity was destroyed by deoxyribonuclease but not by ribonuclease, Pronase, or specific antiserum to the virus. Host mycoplasma cells were only competent for transfection during late-log growth phase. The rates of the establishment of DNase insensitivity of viral DNA transfectants were similar to those of bacteriophage systems. The dose-response curve for transfection suggested that an average of six molecules of DNA must interact with a cell in order to produce one infectious center. Mycoplasmatales virus DNA exhibited a low efficiency of infection; one infectious center required 4 × 105 virus equivalents of DNA.

Keywords: viral DNA isolation, genetic transfer, competence

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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