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. 1972 Dec;69(12):3501–3503. doi: 10.1073/pnas.69.12.3501

Mutants That Produce Nitrogenase in the Presence of Ammonia

Joyce K Gordon 1, Winston J Brill 1
PMCID: PMC389805  PMID: 16592035

Abstract

Mutants of Azotobacter vinelandii that fix N2 in the presence of excess NH4+ have been isolated. A mutant that was unable to synthesize component I and component II of nitrogenase was spontaneously reverted to the N2-fixing phenotype. Of 21 revertants picked, 7 revertants were not as sensitive as the wild type to repression. A derepressed mutant is as sensitive as the wild type to growth inhibition by 2-methylalanine in the presence of glucose.

Keywords: soil fertility, Azotobacter vinelandii, pleiotropic-negative, 2-methylalanine, derepressed

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Selected References

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