Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Dec;69(12):3520–3523. doi: 10.1073/pnas.69.12.3520

Luteinizing Hormone Receptors: Specific Binding of Human Luteinizing Hormone to Homogenates of Luteinized Rat Ovaries

Ching Yung Lee 1, Robert J Ryan 1
PMCID: PMC389811  PMID: 4345503

Abstract

Binding of human [125I]luteinizing hormone to homogenates of luteinized rat ovaries is dependent upon time, temperature, and pH and is saturable. Injection of human chorionic gonadotropin in vivo or addition of unlabeled human chorionic gonadotropin or human or ovine luteinizing hormone in vitro inhibits binding, whereas follicle stimulating hormone or prolactin is without effect. The dissociation constant for binding of luteinizing hormone receptor is 0.79 nM. The number of binding sites is 94 femtomol/mg of wet weight. NaCl, KCl, MgSO4, and CaCl2 at concentrations below 10 mM have no effect on binding, while all salts significantly inhibit binding at 150 mM. Binding of [125I]luteinizing hormone to its receptors is destroyed by proteolytic enzymes and by phospholipase C and D. The total binding activity is quantitatively recovered in the 2000 × g pellet of the homogenate.

Keywords: dissociation constant, number of binding sites

Full text

PDF
3520

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. De Kretser D. M., Catt K. J., Paulsen C. A. Studies on the in vitro testicular binding of iodinated luteinizing hormone in rats. Endocrinology. 1971 Feb;88(2):332–337. doi: 10.1210/endo-88-2-332. [DOI] [PubMed] [Google Scholar]
  2. Dorrington J. H., Baggett B. Adenyl cyclase activity in the rabbit ovary. Endocrinology. 1969 May;84(5):989–996. doi: 10.1210/endo-84-5-989. [DOI] [PubMed] [Google Scholar]
  3. Dufau M. L., Catt K. J., Tsuruhara T. Retention of in vitro biological activities by desialylated human luteinizing hormone and chorionic gonadotropin. Biochem Biophys Res Commun. 1971 Sep;44(5):1022–1029. doi: 10.1016/s0006-291x(71)80188-2. [DOI] [PubMed] [Google Scholar]
  4. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gospodarowicz D. Immunologic and steroidogenic activity of the luteinizing hormone (LH) compared to its two subunits: CI and CII. Endocrinology. 1971 Sep;89(3):669–678. doi: 10.1210/endo-89-3-669. [DOI] [PubMed] [Google Scholar]
  6. Kammerman S., Canfield R. E. The inhibition of binding of iodinated human chorionic gonadotropin to mouse ovary in vitro. Endocrinology. 1972 Feb;90(2):384–389. doi: 10.1210/endo-90-2-384. [DOI] [PubMed] [Google Scholar]
  7. Lee C. Y., Ryan R. J. The uptake of human luteinizing hormone (hLH) by slices of luteinized rat ovaries. Endocrinology. 1971 Dec;89(6):1515–1523. doi: 10.1210/endo-89-6-1515. [DOI] [PubMed] [Google Scholar]
  8. Moudgal N. R., Moyle W. R., Greep R. O. Specific binding of luteinizing hormone to Leydig tumor cells. J Biol Chem. 1971 Aug 25;246(16):4983–4986. [PubMed] [Google Scholar]
  9. Pierce J. G., Bahl O. P., Cornell J. S., Swaminathan N. Biologically active hormones prepared by recombination of the alpha chain of human chorionic gonadotropin and the hormone-specific chain of bovine thyrotropin or of bovine luteinizing hormone. J Biol Chem. 1971 Apr 10;246(7):2321–2324. [PubMed] [Google Scholar]
  10. Rajaniemi H., Vanha-Perttula T. Specific receptor for LH in the ovary: evidence by autoradiography and tissue fractionation. Endocrinology. 1972 Jan;90(1):1–9. doi: 10.1210/endo-90-1-1. [DOI] [PubMed] [Google Scholar]
  11. Sanborn B. M., Rao B. R., Korenman S. G. Interaction of 17 -estradiol and its specific uterine receptor. Evidence for complex kinetic and equilibrium behavior. Biochemistry. 1971 Dec 21;10(26):4955–4962. doi: 10.1021/bi00802a019. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES