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Tumor hypoxia is increased in DKK1 Tg mice and decreased in DKK2 Tg mice compared to wild-type littermates. B16F10 tumor sections of DKK1 Tg, DKK2 Tg, and wild-type littermates were stained with anti-hypoxia-inducing factor-1α (HIF1α) antibody and anti-CD31 antibody. Hypoxic regions were presented (a) and quantified as the ratio of HIF1α+ tumor area to total tumor area (b, c)
