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. 1972 Dec;69(12):3602–3605. doi: 10.1073/pnas.69.12.3602

Release of Polypeptide Chain Initiation Factor IF-2 During Initiation Complex Formation

Arthur H Lockwood 1, Probir Sarkar 1, Umadas Maitra 1
PMCID: PMC389830  PMID: 4566451

Abstract

Polypeptide chain initiation factor IF-2 binds to 30S ribosomal subunits. This binding is enhanced by IF-1 and IF-3. During GTP-dependent formation of a 70S initiation complex, IF-2 is released from the ribosome. During 70S initiation complex formation dependent on the methylene analogue of GTP, GMPPCH2P, IF-2 is not released, but remains bound to the 70S ribosome. This result suggests that IF-2 release requires GTP hydrolysis. In agreement with this presumed requirement, IF-2 functions catalytically with GTP, but stoichiometrically with GMPPCH2P, in bringing about 70S initiation complex formation.

Keywords: protein synthesis, E. coli, GTP hydrolysis, ribosomes, fMet-tRNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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