Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Dec;69(12):3660–3664. doi: 10.1073/pnas.69.12.3660

Purification and Properties of D Protein: A Transcription Factor of Escherichia coli

Sudhamoy Ghosh 1, Harrison Echols 1
PMCID: PMC389843  PMID: 4566455

Abstract

D factor is protein that stimulates DNA transcription in vitro by RNA polymerase. For DNA from bacteriophage λ, we present evidence that D protein interacts with DNA to increase the frequency and improve the specificity of initiation by RNA polymerase.

Keywords: RNA polymerase, bacteriophage λ

Full text

PDF
3660

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberts B. M., Frey L. T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA. Nature. 1970 Sep 26;227(5265):1313–1318. doi: 10.1038/2271313a0. [DOI] [PubMed] [Google Scholar]
  2. Barrett K. J., Gibbs W., Calendar R. A transcribing activity induced by satellite phage P4. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2986–2990. doi: 10.1073/pnas.69.10.2986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blattner F. R., Dahlberg J. E. RNA synthesis startpoints in bacteriophage lambda: are the promoter and operator transcribed? Nat New Biol. 1972 Jun 21;237(77):227–232. doi: 10.1038/newbio237227a0. [DOI] [PubMed] [Google Scholar]
  4. Chamberlin M., McGrath J., Waskell L. New RNA polymerase from Escherichia coli infected with bacteriophage T7. Nature. 1970 Oct 17;228(5268):227–231. doi: 10.1038/228227a0. [DOI] [PubMed] [Google Scholar]
  5. Chen B., de Crombrugghe B., Anderson W. B., Gottesman M. E., Pastan I., Perlman R. L. On the mechanism of action of lac repressor. Nat New Biol. 1971 Sep 15;233(37):67–70. doi: 10.1038/newbio233067a0. [DOI] [PubMed] [Google Scholar]
  6. Davison J., Pilarski L. M., Echols H. A factor that stimulates RNA synthesis by purified RNA polymerase. Proc Natl Acad Sci U S A. 1969 May;63(1):168–174. doi: 10.1073/pnas.63.1.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Echols H. Lysogeny: viral repression and site-specific recombination. Annu Rev Biochem. 1971;40:827–854. doi: 10.1146/annurev.bi.40.070171.004143. [DOI] [PubMed] [Google Scholar]
  8. Echols H., Pilarski L., Xcheng P. Y. In vitro repression of phage lambda DNA transcription by a partially purified repressor from lysogenic cells. Proc Natl Acad Sci U S A. 1968 Mar;59(3):1016–1023. doi: 10.1073/pnas.59.3.1016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eron L., Block R. Mechanism of initiation and repression of in vitro transcription of the lac operon of Escherichia coli. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1828–1832. doi: 10.1073/pnas.68.8.1828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Haseltine W. A. In vitro transcription of Escherichia coli ribosomal RNA genes. Nature. 1972 Feb 11;235(5337):329–333. doi: 10.1038/235329a0. [DOI] [PubMed] [Google Scholar]
  11. Jacquet M., Cukier-Kahn R., Pla J., Gros F. A thermostable protein factor acting on in vitro DNA transcription. Biochem Biophys Res Commun. 1971 Dec 17;45(6):1597–1607. doi: 10.1016/0006-291x(71)90204-x. [DOI] [PubMed] [Google Scholar]
  12. Kuo C. H., August J. T. Histone or bacterial basic protein required for replication of bacteriophage RNA. Nat New Biol. 1972 May 24;237(73):105–108. doi: 10.1038/newbio237105a0. [DOI] [PubMed] [Google Scholar]
  13. Leavitt J. C., Moldave K., Nakada D. Stimulation of in vitro RNA synthesis by ribosomes and ribosomal proteins. J Mol Biol. 1972 Sep 14;70(1):15–40. doi: 10.1016/0022-2836(72)90161-1. [DOI] [PubMed] [Google Scholar]
  14. Losick R. In vitro transcription. Annu Rev Biochem. 1972;41:409–446. doi: 10.1146/annurev.bi.41.070172.002205. [DOI] [PubMed] [Google Scholar]
  15. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  16. Mahadik S. P., Srinivasan P. R. Stimulation of DNA-dependent RNA synthesis by a protein associated with ribosomes. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1898–1901. doi: 10.1073/pnas.68.8.1898. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Murooka Y., Lazzarini R. A. Stimulation of RNA synthesis by two protein factors in extracts of Escherichia coli. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2336–2340. doi: 10.1073/pnas.69.8.2336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ptashne M. Specific binding of the lambda phage repressor to lambda DNA. Nature. 1967 Apr 15;214(5085):232–234. doi: 10.1038/214232a0. [DOI] [PubMed] [Google Scholar]
  19. Riggs A. D., Bourgeois S., Newby R. F., Cohn M. DNA binding of the lac repressor. J Mol Biol. 1968 Jul 14;34(2):365–368. doi: 10.1016/0022-2836(68)90261-1. [DOI] [PubMed] [Google Scholar]
  20. Shih T. Y., Bonner J. Thermal denaturation and template properties of DNA complexes with purified histone fractions. J Mol Biol. 1970 Mar;48(3):469–487. doi: 10.1016/0022-2836(70)90059-8. [DOI] [PubMed] [Google Scholar]
  21. Steinberg R. A., Ptashne M. In vitro repression of RNA synthesis by purified lambda phage repressor. Nat New Biol. 1971 Mar 17;230(11):76–80. doi: 10.1038/newbio230076a0. [DOI] [PubMed] [Google Scholar]
  22. Travers A. A., Kamen R. I., Schleif R. F. Factor necessary for ribosomal RNA synthesis. Nature. 1970 Nov 21;228(5273):748–751. doi: 10.1038/228748a0. [DOI] [PubMed] [Google Scholar]
  23. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  24. Wu A. M., Ghosh S., Echols H. Repression by the cI protein of phage lambda: interaction with RNA polymerase. J Mol Biol. 1972 Jun 28;67(3):423–432. doi: 10.1016/0022-2836(72)90460-3. [DOI] [PubMed] [Google Scholar]
  25. Wu A. M., Ghosh S., Echols H., Spiegelman W. G. Repression by the cI protein of phage lambda: in vitro inhibition of RNA synthesis. J Mol Biol. 1972 Jun 28;67(3):407–421. doi: 10.1016/0022-2836(72)90459-7. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES