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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Dec;69(12):3670–3672. doi: 10.1073/pnas.69.12.3670

Computer Simulation of Fluorescence Depolarization Due to Brownian Motion

Stephen C Harvey 1, Herbert C Cheung 1
PMCID: PMC389845  PMID: 16592039

Abstract

A computer program has been written to simulate the Brownian motion of rigid fluorescent molecules. The time dependence of the fluorescence polarization anisotropy as generated by this simulation is in agreement with that predicted by the recent theoretical treatment of Belford, Belford, and Weber (Proc. Nat. Acad. Sci. USA (1972) 69, 1392-1393). The program thus serves as a verification of their equation. It is being generalized to cover the case of nonrigid molecules.

Keywords: theoretical equation, protein molecules, rigid molecules

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belford G. G., Belford R. L., Weber G. Dynamics of fluorescence polarization in macromolecules. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1392–1393. doi: 10.1073/pnas.69.6.1392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. WEBER G. Rotational Brownian motion and polarization of the fluorescence of solutions. Adv Protein Chem. 1953;8:415–459. doi: 10.1016/s0065-3233(08)60096-0. [DOI] [PubMed] [Google Scholar]

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