Abstract
In vitro assembly of tobacco mosaic virus (TMV) from its constituent RNA and protein occurs in two steps: formation of the initial complex by interaction of the 5′-end of TMV-RNA with 20S protein aggregate, and growth of the helical rod by sequential addition of protein subunits.
Such a process was also observed in the assembly of cucumber green mottle mosaic virus, which is also a rodshaped virus. The 13S virus-protein aggregate, probably a single-ring structure, is needed for formation of the initial complex, instead of the 20S double-ring structure for TMV. The 13S aggregate cannot effect growth of the initial complex to a long infective rod. Rod elongation takes place by the sequential addition of protein subunits.
In general, the assembly of rod-shaped viruses occurs in two steps, and the protein aggregate is essential only for initiation of the reaction, not for rod elongation.
Keywords: protein aggregate, RNA-protein interaction, reconstitution of virus
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Butler P. J., Klug A. Assembly of the particle of tobacco mosaic virus from RNA and disks of protein. Nat New Biol. 1971 Jan 13;229(2):47–50. doi: 10.1038/newbio229047a0. [DOI] [PubMed] [Google Scholar]
- FRAENKEL-CONRAT H. Degradation of tobacco mosaic virus with acetic acid. Virology. 1957 Aug;4(1):1–4. doi: 10.1016/0042-6822(57)90038-7. [DOI] [PubMed] [Google Scholar]
- FRAENKEL-CONRAT H., SINGER B. Reconstitution of tobacco mosaic virus. III. Improved methods and the use of mixed nucleic acids. Biochim Biophys Acta. 1959 Jun;33(2):359–370. doi: 10.1016/0006-3002(59)90126-x. [DOI] [PubMed] [Google Scholar]
- FRAENKEL-CONRAT H., SINGER B., TSUGITA A. Purification of viral RNA by means of bentonite. Virology. 1961 May;14:54–58. doi: 10.1016/0042-6822(61)90131-3. [DOI] [PubMed] [Google Scholar]
- Fraenkel-Conrat H., Williams R. C. RECONSTITUTION OF ACTIVE TOBACCO MOSAIC VIRUS FROM ITS INACTIVE PROTEIN AND NUCLEIC ACID COMPONENTS. Proc Natl Acad Sci U S A. 1955 Oct 15;41(10):690–698. doi: 10.1073/pnas.41.10.690. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nozu Y., Okada Y. Amino acid sequence of a common Japanese strain of tobacco mosaic virus. J Mol Biol. 1968 Aug 14;35(3):643–646. doi: 10.1016/s0022-2836(68)80021-x. [DOI] [PubMed] [Google Scholar]
- Nozu Y., Tochihara H., Komuro Y., Okada Y. Chemical and immunological characterization of cucumber green mottle mosaic virus (watermelon strain) protein. Virology. 1971 Sep;45(3):577–585. doi: 10.1016/0042-6822(71)90173-5. [DOI] [PubMed] [Google Scholar]
- Okada Y., Oashi Y., Ono T., Nozu Y. Sequential reconstitution of tobacco mosaic virus. Virology. 1970 Sep;42(1):243–245. doi: 10.1016/0042-6822(70)90261-8. [DOI] [PubMed] [Google Scholar]
- Richards K. E., Williams R. C. Assembly of tobacco mosaic virus in vitro: effect of state of polymerization of the protein component. Proc Natl Acad Sci U S A. 1972 May;69(5):1121–1124. doi: 10.1073/pnas.69.5.1121. [DOI] [PMC free article] [PubMed] [Google Scholar]