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. 1972 Dec;69(12):3689–3692. doi: 10.1073/pnas.69.12.3689

Crystals of Phosphorylcholine-Binding Fab-Fragments from Mouse Myeloma Proteins: Preparation and X-Ray Analysis

Stuart Rudikoff *, Michael Potter *, David M Segal †, Eduardo A Padlan †, David R Davies †
PMCID: PMC389850  PMID: 4566456

Abstract

Fab-fragments of several phosphorylcholine-binding mouse-myeloma proteins have been prepared by pepsin digestion; two of these, MOPC 167 and McPC 603, gave large crystals from ammonium sulfate solutions. The Fab-fragment from MOPC 167 crystallizes in a hexagonal space group, but does not diffract to a resolution greater than about 8 Å. In contrast, McPC 603 crystals (space group P63) diffract to about 2.7 Å. An isotopic double-labeling technique was developed that demonstrated that the 603 crystals bind 1 mol of hapten per mol of Fab-fragment, but with a binding constant significantly lower than that observed in solution. The findings indicate that a three-dimensional model of this homogeneous antigen-binding immunoglobulin can be constructed. Accordingly, a search for heavy-atom derivatives and determination of the primary structure are in progress.

Keywords: IgA, pepsin fragments, hapten binding, x-ray crystallography

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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