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. 1972 Dec;69(12):3718–3722. doi: 10.1073/pnas.69.12.3718

Perturbed Angular Correlation Study of a Haptenic Molecule

Claude F Meares 1,*, Michael W Sundberg 1, John D Baldeschwieler 1
PMCID: PMC389856  PMID: 16592041

Abstract

The angular correlation of the 173-247 keV gamma-ray cascade after the electron-capture decay of 111In is strongly perturbed when the 1-p-nitrophenyl-ethylenediaminetetraacetate chelate of 111In8+ is added to a solution containing rabbit antibody to dinitrophenyl groups. The radioactive chelate can be displaced by the addition of dinitrophenyllysine or unlabeled chelate. The average association constant between the antibody and the labeled chelate has been estimated from perturbed angular correlation measurements; this value is compared to the results of equilibrium dialysis. These experiments provide good evidence that information concerning macromolecular behavior can be obtained from perturbed angular correlation experiments that use chemically specific labels.

Keywords: rotational label, anti-Dnp, radioactive, 111In

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. EISEN H. N., SISKIND G. W. VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE. Biochemistry. 1964 Jul;3:996–1008. doi: 10.1021/bi00895a027. [DOI] [PubMed] [Google Scholar]
  2. Glass J. C., Graf G. Directional correlation studies of anomalous water in carbonic anhydrase. Nature. 1970 May 16;226(5246):635–636. doi: 10.1038/226635a0. [DOI] [PubMed] [Google Scholar]
  3. Haugland R. P., Stryer L., Stengle T. R., Baldeschwieler J. D. Nuclear magnetic resonance studies of antibody-hapten interactions using a chloride ion probe. Biochemistry. 1967 Feb;6(2):498–502. doi: 10.1021/bi00854a018. [DOI] [PubMed] [Google Scholar]
  4. Leipert T. K., Baldeschwieler J. D., Shirley D. A. Applications of gamma ray angular correlations to the study of biological macromolecules in solution. Nature. 1968 Nov 30;220(5170):907–909. doi: 10.1038/220907a0. [DOI] [PubMed] [Google Scholar]
  5. Meares C. F., Bryant R. G., Baldeschwieler J. D., Shirley D. A. Study of carbonic anhydrase using perturbed angular correlations of gamma radiation. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1155–1161. doi: 10.1073/pnas.64.4.1155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Meares C. F., Westmoreland D. G. The study of biological macromolecules using perturbed angular correlations of gamma radiation. Cold Spring Harb Symp Quant Biol. 1972;36:511–516. doi: 10.1101/sqb.1972.036.01.065. [DOI] [PubMed] [Google Scholar]
  7. Siegel A., Degens E. T. Concentration of dissolved amino acids from saline waters by ligand-exchange chromatography. Science. 1966 Mar 4;151(3714):1098–1101. doi: 10.1126/science.151.3714.1098. [DOI] [PubMed] [Google Scholar]
  8. Stryer L., Griffith O. H. A spin-labeled hapten. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1785–1791. doi: 10.1073/pnas.54.6.1785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Yguerabide J., Epstein H. F., Stryer L. Segmental flexibility in an antibody molecule. J Mol Biol. 1970 Aug;51(3):573–590. doi: 10.1016/0022-2836(70)90009-4. [DOI] [PubMed] [Google Scholar]

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