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. 1972 Dec;69(12):3774–3778. doi: 10.1073/pnas.69.12.3774

Binding of Thymus- and Bone Marrow-Derived Lymphoid Cells to Antigen-Derivatized Fibers

U Rutishauser 1, G M Edelman 1
PMCID: PMC389869  PMID: 4118872

Abstract

Thymus-derived lymphocytes (T cells) and bone marrow-derived lymphocytes (B cells) from mouse spleens bind specifically to antigen-derivatized nylon fibers. The fiber-bound population consisted of about 60%-70% B cells and 30% T cells as determined by cytotoxicity, fluorescence, and antibody-complement binding assays. Essentially all fiber-bound cells were viable and could be accounted for as T or B cells. Enriched populations of T or B cells could be isolated on the fibers by destruction of one or the other cell type with the appropriate antiserum plus complement. T or B cells could also be fractionated according to their relative affinity (or avidity) for a given antigen.

Keywords: mouse spleen, nylon fibers, antiserum, cytotoxicity, fluorescence

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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