Fig. 2.

Protective efficacy of antigen 85A administered i.n. or parenterally. Unimmunized, i.n. or parenterally immunized BALB/c mice were challenged 5 weeks after the last immunization with Mtb i.n. and after further 5 weeks sacrificed for enumeration of lung and spleen (not shown) Mtb CFU. (A) Ad85A was administered i.n. or i.m. without adjuvant. (B) r85A was administered i.n. with cholera toxin or s.c. with MPL. (C) p85A were administered i.n. with cholera toxin or s.c. with MPL. Data from one of three experiments with 5 mice/group are shown. ***p < 0.001, *p < 0.05 compared to naïve animals, one-way ANOVA with Tukey's post test.