Figure 7.

PPARγ pathway negatively regulates HIV replication in T-cells at levels post-entry and prior integration. (A) CD4⁺ T-cells were isolated, activated via TCR and nucleofected with PPARγ vs. NT1 siRNA as described in Figure 6. Cells were exposed to single round HIV-VSVG-GFP at 24 h post-nucleofection and cultured for three days in the presence of IL-2. (B) Shown are levels of integrated HIV-DNA (n = 5). (C) Shown is the frequency and MFI of GFP-expressing cells in one representative subject (upper panels) and statistical analysis in n = 5 different subjects (lower panels). Paired t-Test values are indicated on the graphs.