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. 1983 Dec;80(24):7447–7451. doi: 10.1073/pnas.80.24.7447

Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus.

T Takegami, R J Kuhn, C W Anderson, E Wimmer
PMCID: PMC389968  PMID: 6324172

Abstract

A small nucleotidyl-protein has been synthesized in vitro in a membrane fraction of poliovirus-infected HeLa cells. Analyses of the nucleotides and polypeptide have shown that the nucleotidyl-protein is VPg-pUpU: the genome-linked protein of poliovirion RNA covalently bound to the first two 5'-terminal nucleotides of poliovirus RNA. Synthesis of VPg-pUpU in vitro was sensitive to nonionic detergent. We suggest that VPg-pUpU is part of the initiation complex in poliovirus RNA replication in a membranous environment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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