Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Dec;80(24):7547–7550. doi: 10.1073/pnas.80.24.7547

Increasing the intracellular Na+ concentration induces differentiation in a pre-B lymphocyte cell line.

P M Rosoff, L C Cantley
PMCID: PMC389989  PMID: 6608724

Abstract

The ability of lipopolysaccharide (LPS), a polyclonal B-cell mitogen, to induce differentiation in the pre-B cell tumor line 70Z/3 can be mimicked by drugs that increase the intracellular Na+ concentration. Pharmacologically increasing the cellular Na+ content with monensin, a sodium ionophore, or ouabain, a specific inhibitor of Na+, K+-ATPase, induces surface IgM expression in these cells. We have shown that LPS stimulates uptake of Na+ through an amiloride-sensitive pathway. These results show that the essential action of LPS to induce surface IgM expression is activation of a Na+ uptake system.

Full text

PDF
7547

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aickin C. C., Thomas R. C. An investigation of the ionic mechanism of intracellular pH regulation in mouse soleus muscle fibres. J Physiol. 1977 Dec;273(1):295–316. doi: 10.1113/jphysiol.1977.sp012095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Averdunk R. Early changes of 'leak flux' and the cation content of lymphocytes by concanavalin A. Biochem Biophys Res Commun. 1976 May 3;70(1):101–109. doi: 10.1016/0006-291x(76)91114-1. [DOI] [PubMed] [Google Scholar]
  3. Baer J. E., Jones C. B., Spitzer S. A., Russo H. F. The potassium-sparing and natriuretic activity of N-amidino-3,5-diamino-6-chloropyrazinecarboxamide hydrochloride dihydrate (amiloride hydrochloride). J Pharmacol Exp Ther. 1967 Aug;157(2):472–485. [PubMed] [Google Scholar]
  4. Bernstein A., Hunt D. M., Crichley V., Mak T. W. Induction by ouabain of hemoglobin synthesis in cultured Friend erythroleukemic cells. Cell. 1976 Nov;9(3):375–381. doi: 10.1016/0092-8674(76)90082-9. [DOI] [PubMed] [Google Scholar]
  5. Bridges K., Levenson R., Housman D., Cantley L. Calcium regulates the commitment of murine erythroleukemia cells to terminal erythroid differentiation. J Cell Biol. 1981 Aug;90(2):542–544. doi: 10.1083/jcb.90.2.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Deutsch C., Price M. A. Cell calcium in human peripheral blood lymphocytes and the effect of mitogen. Biochim Biophys Acta. 1982 May 7;687(2):211–218. doi: 10.1016/0005-2736(82)90548-x. [DOI] [PubMed] [Google Scholar]
  7. Deutsch C., Price M. A., Johansson C. A sodium requirement for mitogen-induced proliferation in human peripheral blood lymphocytes. Exp Cell Res. 1981 Dec;136(2):359–369. doi: 10.1016/0014-4827(81)90015-x. [DOI] [PubMed] [Google Scholar]
  8. Deutsch C., Price M. Role of extracellular Na and K in lymphocyte activation. J Cell Physiol. 1982 Oct;113(1):73–79. doi: 10.1002/jcp.1041130113. [DOI] [PubMed] [Google Scholar]
  9. Felber S. M., Brand M. D. Concanavalin A causes an increase in sodium permeability and intracellular sodium content of pig lymphocytes. Biochem J. 1983 Mar 15;210(3):893–897. doi: 10.1042/bj2100893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hesketh T. R., Smith G. A., Moore J. P., Taylor M. V., Metcalfe J. C. Free cytoplasmic calcium concentration and the mitogenic stimulation of lymphocytes. J Biol Chem. 1983 Apr 25;258(8):4876–4882. [PubMed] [Google Scholar]
  11. Kincade P. W. Formation of B lymphocytes in fetal and adult life. Adv Immunol. 1981;31:177–245. doi: 10.1016/s0065-2776(08)60921-9. [DOI] [PubMed] [Google Scholar]
  12. Kishimoto T., Miki Y., Kishi H., Muraguchi A., Kishimoto S., Yamamura Y. IgG induction in a human B cell line by red cell-mediated microinjection of the cytoplasm from T cell factor-stimulated B cells. J Immunol. 1982 Oct;129(4):1367–1371. [PubMed] [Google Scholar]
  13. Koch K. S., Leffert H. L. Increased sodium ion influx is necessary to initiate rat hepatocyte proliferation. Cell. 1979 Sep;18(1):153–163. doi: 10.1016/0092-8674(79)90364-7. [DOI] [PubMed] [Google Scholar]
  14. Levenson R., Housman D., Cantley L. Amiloride inhibits murine erythroleukemia cell differentiation: evidence for a Ca2+ requirement for commitment. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5948–5952. doi: 10.1073/pnas.77.10.5948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Levenson R., Housman D. Commitment: how do cells make the decision to differentiate? Cell. 1981 Jul;25(1):5–6. doi: 10.1016/0092-8674(81)90225-7. [DOI] [PubMed] [Google Scholar]
  16. Maizel A., Sahasrabuddhe C., Mehta S., Morgan J., Lachman L., Ford R. Biochemical separation of a human B cell mitogenic factor. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5998–6002. doi: 10.1073/pnas.79.19.5998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mendoza S. A., Wigglesworth N. M., Pohjanpelto P., Rozengurt E. Na entry and Na-K pump activity in murine, hamster, and human cells--effect of monensin, serum, platelet extract, and viral transformation. J Cell Physiol. 1980 Apr;103(1):17–27. doi: 10.1002/jcp.1041030104. [DOI] [PubMed] [Google Scholar]
  18. Metcalfe J. C., Pozzan T., Smith G. A., Hesketh T. R. A calcium hypothesis for the control of cell growth. Biochem Soc Symp. 1980;45:1–26. [PubMed] [Google Scholar]
  19. Moolenaar W. H., Boonstra J., van der Saag P. T., de Laat S. W. Sodium/proton exchange in mouse neuroblastoma cells. J Biol Chem. 1981 Dec 25;256(24):12883–12887. [PubMed] [Google Scholar]
  20. Moore R. D. Stimulation of Na:H exchange by insulin. Biophys J. 1981 Feb;33(2):203–210. doi: 10.1016/S0006-3495(81)84881-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Paige C. J., Kincade P. W., Ralph P. Murine B cell leukemia line with inducible surface immunoglobulin expression. J Immunol. 1978 Aug;121(2):641–647. [PubMed] [Google Scholar]
  22. Paige C. J., Schreier M. H., Sidman C. L. Mediators from cloned T helper cell lines affect immunoglobulin expression by B cells. Proc Natl Acad Sci U S A. 1982 Aug;79(15):4756–4760. doi: 10.1073/pnas.79.15.4756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pouysségur J., Chambard J. C., Franchi A., Paris S., Van Obberghen-Schilling E. Growth factor activation of an amiloride-sensitive Na+/H+ exchange system in quiescent fibroblasts: coupling to ribosomal protein S6 phosphorylation. Proc Natl Acad Sci U S A. 1982 Jul;79(13):3935–3939. doi: 10.1073/pnas.79.13.3935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ridgway E. B., Gilkey J. C., Jaffe L. F. Free calcium increases explosively in activating medaka eggs. Proc Natl Acad Sci U S A. 1977 Feb;74(2):623–627. doi: 10.1073/pnas.74.2.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rindler M. J., Taub M., Saier M. H., Jr Uptake of 22Na+ by cultured dog kidney cells (MDCK). J Biol Chem. 1979 Nov 25;254(22):11431–11439. [PubMed] [Google Scholar]
  26. Rothenberg P., Glaser L., Schlesinger P., Cassel D. Epidermal growth factor stimulates amiloride-sensitive 22Na+ uptake in A431 cells. Evidence for Na+/H+ exchange. J Biol Chem. 1983 Apr 25;258(8):4883–4889. [PubMed] [Google Scholar]
  27. Rozengurt E., Gelehrter T. D., Legg A., Pettican P. Melittin stimulates Na entry, Na-K pump activity and DNA synthesis in quiescent cultures of mouse cells. Cell. 1981 Mar;23(3):781–788. doi: 10.1016/0092-8674(81)90442-6. [DOI] [PubMed] [Google Scholar]
  28. Rozengurt E. Stimulation of Na influx, Na-K pump activity and DNA synthesis in quiescent cultured cells. Adv Enzyme Regul. 1980;19:61–85. doi: 10.1016/0065-2571(81)90009-1. [DOI] [PubMed] [Google Scholar]
  29. Schuldiner S., Rozengurt E. Na+/H+ antiport in Swiss 3T3 cells: mitogenic stimulation leads to cytoplasmic alkalinization. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7778–7782. doi: 10.1073/pnas.79.24.7778. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Segel G. B., Simon W., Lichtman M. A. Regulation of sodium and potassium transport in phytohemagglutinin-stimulated human blood lymphocytes. J Clin Invest. 1979 Sep;64(3):834–841. doi: 10.1172/JCI109531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Smith R. L., Macara I. G., Levenson R., Housman D., Cantley L. Evidence that a Na+/Ca2+ antiport system regulates murine erythroleukemia cell differentiation. J Biol Chem. 1982 Jan 25;257(2):773–780. [PubMed] [Google Scholar]
  32. Steinhardt R. A., Epel D. Activation of sea-urchin eggs by a calcium ionophore. Proc Natl Acad Sci U S A. 1974 May;71(5):1915–1919. doi: 10.1073/pnas.71.5.1915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Tsien R. Y., Pozzan T., Rink T. J. T-cell mitogens cause early changes in cytoplasmic free Ca2+ and membrane potential in lymphocytes. Nature. 1982 Jan 7;295(5844):68–71. doi: 10.1038/295068a0. [DOI] [PubMed] [Google Scholar]
  34. Villereal M. L. Sodium fluxes in human fibroblasts: effect of serum, Ca+2, and amiloride. J Cell Physiol. 1981 Jun;107(3):359–369. doi: 10.1002/jcp.1041070307. [DOI] [PubMed] [Google Scholar]
  35. Wysocki L. J., Sato V. L. "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844–2848. doi: 10.1073/pnas.75.6.2844. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES