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. 1983 Dec;80(23):7065–7069. doi: 10.1073/pnas.80.23.7065

A frameshift mutation affecting the carboxyl terminus of the simian virus 40 large tumor antigen results in a replication- and transformation-defective virus.

E D Lewis, S Chen, A Kumar, G Blanck, R E Pollack, J L Manley
PMCID: PMC389993  PMID: 6316342

Abstract

We have constructed a frameshift mutation in the simian virus 40 early region using a novel method of oligonucleotide-directed mutagenesis. The mutated DNA specifies an 84,000-dalton large tumor antigen that consists of approximately equal to 75,000 daltons encoded by the wild-type reading frame and 9,000 daltons, by the alternative reading frame (wild-type large tumor antigen is approximately equal to 82,000 daltons). The frameshifted carboxyl terminus of the protein bears a strong similarity to the same region of polyoma virus middle-sized tumor antigen. We have found that the mutant DNA is unable to replicate when introduced into permissive monkey cells and incapable of transforming nonpermissive mouse cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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